英文名称： Yeast Lytic Enzyme - Zymolyase™
| • | Lyophilized Zymolyase available. |
| • | Optimized concentration (5 U/µl) for maximum lytic activity. |
| • | DNase/RNase-Free |
| • | Enzyme Activities: ß-1,3-glucan laminaripentaohydrolase and b-1,3-glucanase (trace amount of protease, ca. 1.5 units per 10 µl). DNase and RNase: none detectable. |
| • | Protein Content: ~10-15 mg/ml |
| • | Deactivation: Lytic activity is lost in 5 minutes at 60°C. |
| • | Unit Definition: One lytic unit is defined as a 10% decrease in O.D. at 800 nm for 30 minutes. |
| • | Assay Condition: 50 mM potassium phosphate, pH 7.5, 10 mM 2-mercaptoethanol in 1 ml yeast cell suspension of an O.D. of 0.8 to 1.0 at 800 nm. |
| • | Concentration: 5 U/µl |
| • | Storage/Stability: Zymolyase is stable for over 1 year at -20°C or many years below -70°C. |
Digestion of yeast and fungal cell walls is necessary for many experimental procedures including spheroplasting, immunofluorescence, transformation, protein purification, and others. The use of lytic enzymes like Zymolyase is routinely used for digestion. Zymo Research’s liquid and lyophilized forms of the yeast lytic enzyme Zymolyase is prepared from Arthrobacter luteus. The buffer used to solubilize the Zymolyase is optimized to confer maximal levels of enzymatic activity. The main enzymatic activities are ß-1,3-glucanase and ß-1,3-glucan laminaripentaohydrolase, which hydrolyze glucose polymers at the ß-1,3-glucan linkages releasing laminaripentaose as the principal product.
ORDREING INFORMATION
Product Catalog No. Size Price
Zymolyase E1004 1,000 Units
E1005 2,000 Units
R-Zymolyase E1006 1,000 Units
(includes 500µl storage buffer)