Features PCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume• Two applications in one kit – one buffer with optimal performance for both applications• High recoveries for fragments down to 50 bp• Minimized elution volume of 15 μL – highly concentrated DNA• Binding buffer with pH indicator• Separate buffers for single stranded DNA or SDS containing samples available• Suitable for all gel buffer systems (e.g., TAE, TBE)• Also available for QIAcube® (please see "Ordering information" and contact Technical Service at techbio@mn-net.com for more information).Technology Silica-membrane technologyFormat Mini spin columnsSample material < 400 μL PCR reaction mixture < 400 mg TAE / TBE agarose gelFragment size 50 bp–approx. 20 kbpTypical recovery 70–95%A260/A280 1.8–1.9Elution volume 15–30 μLPreparation time 10 min/6 prepsBinding capacity 25 μgApplications• Purification of PCR products• Extraction of DNA from agarose gels• DNA extraction from polyacrylamide gels• Clean-up of single stranded DNA and RNA extraction from agarose gels (Binding Buffer NTC required, not included in the kit, see “Ordering information”)• Clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the kit, see “Ordering information”)• Typical downstream applications: cloning, sequencing, PCR, restriction analysis• NucleoSpin® Gel and PCR Clean-up ensures complete removal of contaminations such as:- nucleotides, primers- enzymes- mineral oil- PCR additives (e.g., salts, betaine, DMSO)- detergents (e.g., Tween 20, Triton X-100)- dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti®-Safe GelStain, DNA SafeStain) Ordering informationProduct PrepsSpecification REFNucleoSpin® Gel and PCR Clean-up 10NucleoSpin® Gel and PCR Clean-up Columns, Collection Tubes (2 mL), buffers 740609.1050as above 740609.50250as above 740609.250240Kit for use on QIAcube®* NucleoSpin® Gel and PCR Clean-up Columns, buffers 740609.240CProduct accessories Pack ofSpecification REFBuffer NTB 150 mLfor clean-up of SDS-containing samples 740595.150Buffer NTC 100 mLfor clean-up of single stranded DNA