pCMVTNT™ Vector

pCMVTNTTM Vector(a,b)和pTNTTM Vector(b)专为在体内和体外表达系统中方便表达克隆基因设计。SP6和T7聚合酶启动子前后相接，毗临多克隆位点，这种设计允许基于SP6和T7偶联的体外转录/翻译
系统进行基因表达。RNA噬菌体启动子的存在可以满足高效的RNA体外合成需要。两种载体都带有5`端b球蛋白前导序列，据报道该序列可增强某些基因的体外表达(1,2)。对于体内蛋白表达，载体含有一个CMV增强子/启动子区域，在多种细胞系中该区域可保证稳定的组成型表达(3)。b球蛋白/IgG嵌合的内含子位于CMV增强/启动子的下游，晚期SV40多聚腺苷酸化位点位于多克隆位点的下游。

特点
  • 体内表达：在多种细胞系中，CMV增强/启动子区域可保证稳定的组成型表达。
  • 灵活：两个载体含有一前一后的SP6和T7噬菌体启动子，可满足体外翻译或转录系统的要求。

操作手册

pCMVTNT™ Vector Technical Bulletin TB305
pTNT™ Vector Technical Bulletin          TB304

储存条件：储存于-20℃。

GenBank®/EMBL Accession Numbers: Cat.# L5620, AF477200;Cat.# L5610, AF479322.

参考文献
1. Falcone, D. and Andrews, D.W. (1991) Mol. Cell. Biol. 11, 2656–64.
2. Annweiler, A. et al. (1991) Nucl. Acids Res. 19, 3750.
3. Schmidt, E.V. et al. (1990) Mol. Cell. Biol. 10, 4406–11.
4. Brinster, R.L. et al. (1988) Proc. Natl. Acad. Sci. USA 85, 836–40.
(a) The CMV promoter and its use are covered under U.S. Pat. Nos. 5,168,062 and 5,385,839
owned by the University of Iowa Research Foundation, Iowa City, Iowa, and licensed FOR
RESEARCH USE ONLY. Research Use includes contract research for which monetary or
other consideration may be received. Other commercial users must obtain a license to these
patents directly from the University of Iowa Research Foundation.
(b) Licensed for research and laboratory use only under U.S. Pat. No. 5,547,862.

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