Cyber Green is a sensitive fluorescent nucleic acid gel stain for detecting nucleic acids in agarose and polyacrylamide gels. Cyber Green stain exhibits exceptional affinity for DNA and a large fluorescence enhancement upon binding to DNA, at least an order of magnitude greater than that of ethidium bromide when detected by photography. With a standard 300 nm UV transilluminator and photographic detection, as little as 60 pg dsDNA per band can be detected with Cyber Green stain. Cyber Green nucleic acid gel stain is nearly two orders of magnitude more sensitive than ethidium bromide for staining oligonucleotides in gels. Our Gelite Green Nucleic Acid Gel Staining Gel Kit includes our Cyber Green nucleic acid stain with an optimized and robust protocol. It provides a convenient solution for staining nucleic acid samples in gels.