AAT-Screen Quest Fluo-4钙检测试剂盒 *1% FBS生长培养基*
英文名称： Screen Quest Rhod-4 No Wash Calcium Assay Kit *1% FBS Growth Medium* *10x10 plates*
型号:null    产品货号： AAT-36335
价格:请致电：010-57128832,18610462672
品牌: 美国

le in two formats. The two formats are: aligned or non-aligned. The non-aligned Scaffolds are fabricated from spun fibers using a non-woven textile process, are sold as sheets 4 cm square and 2 mm thick and resemble felt. Further modifications of the basic scaffold material can be conducted in the laboratory to enhance cell attachment and growth5,6, 11 or to modify the physical properties and resorption rate of the non-aligned scaffold.10 Standard aligned scaffolds are also fabricated from spun fibers and are available in sheets 4 cm square and .5 mm thick, as well as tubes 4 mm in diameter and 4 cm long. A variety of custom three-dimensional shapes and tubular structures can be made with the aligned scaffolding. Custom sheets can vary in thickness from 50 to 2000 microns. Thicker structures can be made at a premium. The main advantage of the aligned scaffold is that structures, including tubing, are seamless, other scaffolds must be sutured.

EQUL.COM provides the scaffold as non-sterile. The scaffolds are packaged in pouches flushed with an inert gas and containing a desiccant pack. Upon opening, the scaffolds should be thoroughly washed in 100% isopropyl alcohol (IPA), followed by washing in sterile distilled water. In many applications, this washing procedure provides adequate sterilization and the scaffolds can be immediately incubated in media. If validated sterility is desired, the scaffolds should be washed thoroughly in IPA, drained and dried in a desiccated oven at 30 C for 30 minutes. Scaffolds can then be sterilized by ethylene oxide. The temperature should not exceed 30 C during the ethylene oxide sterilization cycle. E-Beam and Gamma sterilization may also be used, however, some deterioration of polymer properties will occur using these methods. These effects are minimized if the scaffold is kept at 0 C or below during irradiation. The scaffold should not be autoclaved.

Custom scaffolds made from polylactide (PLLA), PGA/PLLA copolymers and other synthetic resorbable polymers are available upon request. Please e-mail us at info@SCIDEMO.COM to inquire.

REFERENCES
1. Vacanti, J., Transplantation Society Abstracts, 1998 Meeting, Montreal
2. Ferber, D., Review Article, Science, Vol. 284, April 16, 1999, p. 422
3. Freed, L. et al, Biotechnology, Vol 12, July 1994, p. 689
4. Niklason, L. et al, Science, Vol 284, April 16, 1999, p. 489
5. Kim, B.S. et al, Biotechnol. Bioeng., (1998) 57(1), pp. 46-54
6. Kim, T.H., et al, Tissue Eng., (1997), 3(3), pp. 303-308
7. Vunjak-Novakovic, G. et al, J. Serb. Chem. Soc. (1997), 62(9), pp 787-799
8. Holder, W.D., et al, Tissue Eng., (1997), 3(2), pp 149-160
9. Grande, D. A. et al, J. Biomed. Mater. Res. , (1997), 34(2), pp 211-220
10. Mooney, D.J. et al, Biomaterials, (1996), 17(2), pp115-124
11. Gao, J. et al, J. Biomed. Mater. Res. , 42, 417 (1998).