英文名称： His GraviTrap
His GraviTrap/His GraviTrap Kit - Gravity-flow Purification
| TECHNICAL SPECIFICATIONS | |
| Medium | Ni Sepharose 6 Fast Flow |
| Average particle size | 90 μm |
| Bed volume | 1 ml |
| Column material | Polypropylene barrel, polyethylene frits |
| Protein binding capacity* | Approx. 40 mg histidine-tagged protein/column |
| Compatibility during use | Stable in all commonly used buffers, reducing agents, denaturing agents and detergents, see Introduction to Tagged Protein Purification |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| * Binding capacity is protein-to-protein dependent. | |
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Customer feedback on Ni Sepharose 6 Fast Flow.
His GraviTrap columns
His GraviTrap™ is designed for fast and simple purification of histidine-tagged* proteins using gravity flow.
Both clarified and unclarified sample can be applied to the column and special column frits protect the medium from running dry during purification. A typical purification run on His GraviTrap™ is performed in approximately 30 min.
His GraviTrap™ connected to LabMate™ PD-10 Buffer Reservoir for convenient equilibration, sample application and wash. For optimal performance, use buffers prepared from His Buffer Kit.
His GraviTrap/His GraviTrap Kit - Gravity-flow Purification
Technical Information
The ready to use column is prepacked with Ni Sepharose™ 6 Fast Flow, which has a high protein binding capacity, low nickel-ion leakage, and is compatible with denaturing and reducing agents, as well a wide range of additives¾see Ni Sepharose™ High Performance - High-performance Purification for more information.
His GraviTrap™ columns are delivered in a package that can be converted into a column stand to simplify purification. LabMate PD-10 Buffer Reservoir can be connected to the columns for convenient handling of sample volumes above 10 ml.
For optimal performance, use His GraviTrap with buffers prepared from His Buffer Kit, see His Buffer Kit - Convenient, Pre-made Buffers.
His GraviTrap Kit
The benefits of His GraviTrap™ and His Buffer Kit are combined in His GraviTrap Kit, which contains two packs of His GraviTrap and one pack of His Buffer Kit. His GraviTrap Kit contains columns and buffers for 20 purifications.
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His GraviTrap™ columns allow direct purification of unclarified cell lysates. This shortens the overall time for purification of histidine-tagged proteins, which minimizes degradation and oxidation of sensitive target proteins.