Product Information

Order ID	Name	Description
BiovectorPCAMBIA2201	pCAMBIA2201	pCAMBIA1304,DNA. 15uL,KanR.Storage:-20℃

Transformation of plasmid DNA to competent E. Coli cells

1. Thaw competent cells on ice. 20–200µL per tube
2. Add max. 1-3µL of plasmid
3. Mix very gently!
4. Incubate the tubes on ice for 30 min
5. Heat shock the cells for 45 sec to 2 min at 42°C
6. Place the tubes immediately on ice for at least 2 min
7. Add 800µL of SOC medium to each tube
8. Incubate for 1 hour at 37°C and shake vigorously
9. Spin down briefly and remove most supernatants
10. Resuspend cell pellet with the rest SOC medium in the tube by pipetting
11. Plate out the suspension on a LB agar plate containing the appropriate antibiotic. Incubate the plates overnight at 37°C

载体信息：

质粒类型:	植物双元表达载体
克隆方法:	多克隆位点，限制性内切酶
载体大小:	11785 bp
载体抗性:	Chloramphenicol

中国质粒载体菌株细胞株基因库-Biovector Inc.国家典型培养物保藏中心NTCC，可提供数万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真核表达及纯化、病毒包装、基因沉默RNAi等服务。作为美国质粒载体菌株细胞株基因保藏中心中国分库及唯一办事处，Biovector Inc.可为您提供便捷一站式的产品进***务，到货快捷，步骤简便。 

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