Product List

Order ID	Name	Description
Biovector-01128569	pCAMBIA3300	pCAMBIA3300,KanR,in E.coli. Storage:4℃

Recovery

1.Obtain an LB agar plate with the appropriate antibiotic.

2.Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)

3.Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.

4.Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2.

5.Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3.

6.Grow overnight in a 37 ^o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).

7.In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies.

载体信息：

质粒类型:	植物双元表达载体
克隆方法:	多克隆位点，限制性内切酶
载体大小:	8428 bp
载体抗性:	Kanamycin (卡那霉素)

中国质粒载体菌株细胞株基因库-Biovector Inc.国家典型培养物保藏中心NTCC，可提供数万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真核表达及纯化、病毒包装、基因沉默RNAi等服务。作为美国质粒载体菌株细胞株基因保藏中心中国分库及唯一办事处，Biovector Inc.可为您提供便捷一站式的产品进***务，到货快捷，步骤简便。 

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