(基于转座酶的超快速DNA文库构建体系)TruePrepTMDNA Library Prep Kit V2for Illumina
英文名称: TruePrepTMDNA Library Prep Kit V2for Illumina
型号:null    产品货号: TD503-01/02
价格:请致电:010-57128832,18610462672
品牌: 江苏南京

 基于转座酶的超快速DNA文库构建体系

TruePrepTM DNA Library Prep Kit V2 for Illumina®
 

● 单个样品建库时间少于90分钟

● 单管酶促反应,片段化和加接头反应同时进行,无需使用机械方法(超声、雾化等)进行片段化

● 文库扩增专用聚合酶,配以精心优化的反应缓冲,最大程度上保证了文库扩增的高效性和均一性

● 建库起始DNA量最低仅需1 ng

● 根据起始DNA量分类,提供共计3种不同规格试剂盒

● 提供96种双端index组合

 

图1. TruePrepTM超快速建库流程与传统建库流程耗时对比。 TruePrepTM建库系统将繁琐的DNA处理流程简化为一步10 min孵育,将单个文库构建时间缩短至90 min之内,使得单人单日可以达到96个文库的建库通量。

 


      TruePrepTM DNA Library Prep Kit V2 for Illumina®是针对Illumina高通量测序平台定向开发的专用试剂盒。使用该试剂盒可以将DNA制备成Illumthesis, CPLVSHL-13KM-O)

> 200 ug blue or dry ice -20℃ 10-14 weeks
CPLVSHL-27K-O 27K shRNA Library Design and Synthesis none blue or dry ice -20℃ na
CPLVSHL-27KM-O Design and Synthesis of Multiple shRNA Libraries from one 27K pool none blue or dry ice -20℃ na
CPLVSHL-27K-P 27K shRNA Library Construction (must also order Library Synthesis, CPLVSHL-27K-O) > 200 ug blue or dry ice -20℃ 10-14 weeks
CPLVSHL-27Kd4-P Construction of Four shRNA Libraries from One 27K oligo pool. (order with Library Synthesis, CPLVSHL-27KM-O) > 200 ug blue or dry ice -20℃ 10-14 weeks
CPLVSHL-27Kd3-P Construction of Three shRNA Libraries from One 27K oligo pool. (order with Library Synthesis, CPLVSHL-27KM-O) > 200 ug blue or dry ice -20℃ 10-14 weeks
CPLVSHL-27Kd2-P Construction of Two shRNA Libraries from One 27K oligo pool. (order with Library Synthesis, CPLVSHL-27KM-O) > 200 ug blue or dry ice -20℃ 10-14 weeks
CPLVSHL-55K-O 55K shRNA Library Design and Synthesis none blue or dry ice -20℃ na
CPLVSHL-55KM-O Design and Synthesis of Multiple shRNA Libraries from one 55K pool none blue or dry ice -20℃ na
CPLVSHL-55K-P 55K shRNA Library Construction (must also order Library Synthesis, CPLVSHL-55K-O) > 200 ug blue or dry ice -20℃ 10-14 weeks
CPLVSHL-55Kd4-P Construction of Four shRNA Libraries from One 55K oligo pool. (order with Library Design and Synthesis for new libraries, CPLVSHL-55KM-O) > 200 ug blue or dry ice -20℃ 10-14 weeks
CPLVSHL-55Kd3-P Construction of Three shRNA Libraries from One 55K oligo pool. (order with Library Design and Synthesis for new libraries, CPLVSHL-55KM-O) > 200 ug blue or dry ice -20℃ 10-14 weeks
CPLVSHL-55Kd2-P Construction of Two shRNA Libraries from One 55K oligo pool. (order with Library Design and Synthesis for new libraries, CPLVSHL-55KM-O) > 200 ug blue or dry ice -20℃ 10-14 weeks
CPLVSHL-DUAL-P Custom Combinatorial shRNA Library Made with Dual shRNA Expression Vector (up to 200 shRNAs) > 200 ug blue or dry ice -20℃ 10-14 weeks
CPLVO-CUST-P Non-Standard Custom Library Construction > 200 ug blue or dry ice -20℃ 10-14 weeks
Custom Peptide Libraries          
CPLVPL-6K-O 6.5K Peptide Library Design and Synthesis > 5 pmol blue or dry ice -20℃ 10-12 weeks
CPLVPL-6K-P 6.5K Peptide Library Construction (order with Library Synthesis, CPLVPL-6K-O) > 200 ug blue or dry ice -20℃ 10-12 weeks
CPLVPL-13K-O 13K Peptide Library Design and Synthesis > 5 pmol blue or dry ice -20℃ 10-12 weeks
CPLVPL-13K-P 13K Peptide Library Construction (order with Library Synthesis, CPLVPL-13K-O) > 200 ug blue or dry ice -20℃ 10-12 weeks
CPLVPL-27K-O 27K Peptide Library Design and Synthesis > 5 pmol blue or dry ice -20℃ 10-12 weeks
CPLVPL-27K-P 27K Peptide Library Construction (must also order Library Synthesis, CPLVPL-27K-O) > 200 ug blue or dry ice -20℃ 10-12 weeks
CPLVPL-55K-O 55K Peptide Library Design and Synthesis > 5 pmol blue or dry ice -20℃ 10-12 weeks
CPLVPL-55K-P 55K Peptide Library Construction (must also order Library Synthesis, CPLVPL-55K-O) > 200 ug blue or dry ice -20℃ 10-12 weeks
Packaging Service          
CLVP-V Single Construct Lentiviral Packaging Service (not custom construct) >10^7 TU dry ice -80℃ 2-4 weeks
CLVP-VS Lentiviral Packaging of Custom-Made shRNA Constructs (ordered with shRNA construction) >10^7 TU dry ice -80℃ 2-4 weeks
CLVP-1108 Lentiviral Packaging Service of single construct 2 ml (1 x 10^8 ifu/ml) dry ice -80℃ 3 – 5 weeks
CLVP-5108 Lentiviral Packaging Service of single construct 5x10^8TU (500 ul @ 10^9 ifu/ml) dry ice -80℃ 3 – 5 weeks
CLVP-1109 Lentiviral Packaging Service of single construct >1 x 10^9 dry ice -80℃ 3 – 5 weeks
CLVP-5E7 Lentiviral Library Packaging, small scale (for libraries <6,500 constructs) > 5 x 10^7 ifu dry ice -80℃ 3 – 5 weeks
CLVP-1E8 Lentiviral Library Packaging, 10^8 TU > 1 x 10^8 ifu dry ice -80℃ 3 – 5 weeks
CLVP-2E8 Lentiviral Library Packaging, Standard > 2 x 10^8 ifu dry ice -80℃ 3 – 5 weeks
CLVP-LGLIB Lentiviral Library Packaging, Large Scale > 1 x 10^9 ifu dry ice -80℃ 3 – 5 weeks


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