pPICZ B载体酵母表达载体系统
英文名称： pPICZ B载体
型号:null
价格:请致电：010-57128832,18610462672
品牌: 国外
试剂级别： 分子生物学级

pPICZ B载体酵母表达载体系统质粒图谱、序列、价格、抗性、大小等基本信息-欢迎咨询伟通生物 
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(HD)载体腺病毒载体系统pShuttle-CMV载体pShuttle载体pAdTrack-CMVpAdTrackpAdEasy-1BJ5183菌株AdEasy-1菌株腺病毒载体系统pAd/BLOCK-iT-DEST RNAi Gateway VectorpDC315载体pBHGloxdelE13cre载体腺相关病毒载体系统pAAV-MCS载体pAAV-RC载体pHelper载体pAAV-LacZ载体pAAV-IRES-hrGFP载体pCMV-MCS载体RNAi逆转录病毒载体系统RNAi-Ready pSIREN-RetroQ载体RNAi-Ready pSIREN-RetroQ-DsRed-Express载体RNAi-Ready pSIREN-RetroQ-ZsGreen（Luciferase shRNA Annealed Oligonucleotide），载体pSilencer5.1-U6-Retro载体pSilencer5.1-H1-Retro载体逆转录病毒表达载体系统pRetroX-IRES-ZsGreen1载体pRetroX-IRES-DsRed Express载体pLNCX2载体pQCXIH载体pMSCVpuro载体pSuper retro puro载体pSuper retro GFP-neo载体pBabe puro载体pCMV-Gag-pol载体pCMV-VSV-G载体pCL Ampho载体pVPack-GP载体pCMV VSV G载体RNAi慢病毒载体系统pSicoR载体pSicoR pGK puro载体pLentilox 3.7载体pLKO.1 puro载体pLKO.3G载体pPRIME-TET-GFP-FF3载体pPRIME-TREX-GFP-FF3载体pLVX-shRNA1载体pLVX-shRNA2载体pSIH1-H1-CoGFP载体pGIPZ empty载体pGIPZ control载体pTRIPZ empty载体pTRIPZ control载体pLVX-DsRed-Monomer-N1载体pLVX-AcGFP1-N1载体pLVX-IRES-ZsGreen1载体pLVX-IRES-neo载体pLVX-IRES-td 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protein载体mAmetrine载体启动子增强子报告载体系统pGL3-basic载体pGL3-control载体pGL3-enhancer载体pGL3-promoter载体pRL-TK载体pRL-CMV载体pRL-SV40载体pGluc-basic载体原核表达载体系统pET-11a(+)载体pET-17b(+)载体pET-19b(+)载体pET-20b(+)载体pET-21a(+)载体pET-22b(+)载体pET-26b(+)载体pET-27b(+)载体pET-28a(+)载体pET-28b(+)载体pET-29a(+)载体pET-30a(+)载体pET-32a(+)载体pET-39b(+)载体pET-40b(+)载体pET-42a(+)载体pET49b(+)载体pET50b(+)载体pETduet-1载体pETduet-1载体pRSFDuet-1载体pCDFDuet-1载体BL21菌株BL21(DE3)菌株BL21(DE3)plysS菌株Rosetta(DE3) 菌株Rosetta-gami 2(DE3)pLysS，菌株Rosetta-gami B(DE3)pLysS菌株BLR(DE3)菌株BL21(DE3)gold菌株Rosetta Blue(DE3) 菌株Rosetta(DE3)plysS菌株Rosetta-gami(DE3)菌株Origami(DE3)菌株OrigamiB(DE3)菌株pCold I载体pCold TF载体pMAL-c2x载体pMAL-c4x载体pMAL-p4x载体pLLP-OmpA载体pLLP-STII载体pMBP-P载体pMBP-C载体pET-GST载体pET-Trx载体pET-His载体pTrc-CKS载体pET-DsbA载体BL21 codonplus（DE3）RIPL菌株pRSET A载体BL21(AI)菌株pQE30载体M15菌株pTWIN1载体pTYB11载体pMXB10载体pTXB1载体ER2566菌株pGEX-4T-1载体pGEX-5X-2载体pGEX-6P-1载体pGEX-2TK载体pBADHis A载体pBADHis B载体pBADHis C载体C43(DE3)菌株pBV220载体pBluescript II SK(+)载体pG-KJE8载体pGro7载体pKJE7载体pG-Tf2载体pTf16载体pOG44载体pORF-lacZ载体pORF-HSV1tk载体pUC18载体pUC19载体pBR322载体pSP64载体pSP73载体SuperCos1pSIM5载体pSIM6载体pKD3载体pKD4载体pKD20载体pKD46载体PCP20（30度）载体BW25113菌株克隆菌株DH5α菌株JM110菌株TOP10菌株XL2-Blue MRF?株Mach1 T1菌株OmniMAX2 T1Phage-Resistant Cells菌株XL10 Gold菌株EZ10菌株DH10B菌株SURE菌株pCDH-EF1-MCS-T2A-Puro载体pLVTHM载体pLOX-CW-CRE载体pP-αSUMO3载体SUMO protease 2菌株pCDH-EF1-MCS-T2A-Puro载体pBI CMV1载体BL21gold(DE3) plysS菌株pE-SUMO载体pMG36e载体MG1363菌株Y1H GoldpGADT7-RecpX334pX335pCas-GiduepShuttle-IRES-hrGFP2pacAd5 CMVpacAd5 9.2-100pacAd5 CMV-GFPpDC311pDC312pDC316pacAd5 CMV-nt LacZ ControlpAd/CMV/V5-DESTpAd/PL-DESTpCL AmphopSuper retro puropMXs-hOct4pMXs-hSox2pMXs-hKlf4pMXs-hC-Mycp10A1pAmphopEcopVSV-GpQCLINpMSCV neopLVX-IRES-mCherrypLVX-IRES-neopCDH-CMV-MCS-EF1-puropCDH-EF1-MCS-T2A-PuropLVTHMFUW-tetO-hOCT4FUW-tetO-hSOX2FUW-tetO-hKLF4FUW-tetO-hMYCLeGO-iC2pLOX-CW-CREpLKO-puro Turbo GFPpLVX-EF1a-IRES puropCDH-MCS-T2A-Puro-MSCVpLKO-Tet-OnpLVX-AcGFP1-C1pLL3.7-RFPHDM-PM2HDM-Rev1bHDM-Tat1bHDM-VSV-GpsiLV-H1Plv-pk-FIVPlv-pk-VSGpE2F-TA-LucpFA2-Elk1pFC-MEKKpGL4.79pGL4.17pGL4.15pRNATin-H1.2/NeopRNATin-H1.2/HygropRNATin-H1.2/RetropRNA-H1.1/AdenopSingle-tTS-shRNApMIR-REPORT β-gal ControlpGAPZα BpGAPZα CpPinkα HCpENTR/U6pENTR/H1/TOpCLIP fpCLIP f -NK1RpEF4/V5 HisApcDNA4/V5-His ApDEST 10pIEX-Bac1pZeoSV2pCMV-Tag3BpLIVEpFRT/lacZeopcDNA5/FRTpcDNA5/FRT/CATpIEX-1pIRES2-DsRed expresspET41a(+)pET43.1a(+)pET15b(+)pET31b(+)pET48b(+)Turner（DE3）pCold IIpCold IIIpEcoli-6xHN-GFPuvC41(DE3)pCOLA duet-1ArcticExpress (DE3)RPpET52b(+)pQE32pET25b(+)pET35b(+)Rosetta-gami B(DE3)TB1(DE3)C41(DE3) pLysSC43(DE3) pLysSpBAD24pMAL-p5gpSV-β-GalactosidasepKD13DH5α λpir
 

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VCSM13

Helper Phage VCSM13, 100uL.Store -20°C

Description

Interference-resistant helper phage (6 kb, single stranded), Derived from M13 K07 mutant, Carries kanamycin-resistance gene in IG region, High rescue efficiencies with kanamycin selection

Phage Amplification

If the titer drops over time, grow up XL1-Blue cells in 10 ml of media to an OD600 of 0.3 and add VCSM13 helper phage at a multiplicity of infection of 20:1 (phage-to-cell-ratio). Grow at 37°C for 30 minutes and then add kanamycin to a final concentration of 70 µg/ml. Continue growing at 37°C for 8 hours, heat to 65°C for 15 minutes and spin down the cell debris.

Titer of the supernatant should be between 1 × 1011 and 1 × 1012 pfu/ml. For further details about phage amplification or titering, please see Reference 1.

Reference 1.  

Sambrook, J., Fritsch, E. F., and Maniatis, T., eds. (1989) “Molecular Cloning: A Laboratory Manual,” Second Edition, 4.21-4.23. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

Product Information

Order ID

Name

Description

Biovector-01128569

XL1-Blue

TG1

XL1-Blue,200uL,TetR. Storage:4℃

TG1,200uL,TetR. Storage:4℃

Genotype:

XL1-Blue:

recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F´ proAB

lacIqZΔM15 Tn10 (Tetr)]

TG1:

F’[traD36 lacIq ∆(lacZ) M15 proA+B+] glnV (supE) thi-1 ∆(mcrB-hsdSM)5 (rK- mK- McrB-) thi ∆(lac-proAB)

Description: 

The XL1-Blue strain allows blue-white color screening for recombinant plasmids and is an excellent host strain for routine cloning applications using plasmid or lambda vectors.

XL-1 cells are tetracycline resistant. XL1-Blue cells are endonuclease (endA) deficient, which greatly improves the quality of miniprep DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned DNA by the EcoK endonuclease system. The lacIqZΔM15 gene on the F´ episome allows blue-white color screening.

TG1 strain is particularly useful for phage display protein expression. TG1 cells are also suitable for M13 phage work, general cloning, blue/white screening and protein expression.

Recovery

1.          Obtain an LB agar plate with the appropriate antibiotic.

2.          Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)

3.          Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.

4.          Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2.

5.          Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3.

6.          Grow overnight in a 37 o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).

7.          In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies.