载体基本信息

出品公司:	Invitrogen
载体名称:	pCEP4
质粒类型:	哺乳动物细胞表达载体
启动子:	CMV
表达水平:	高
克隆方法:	多克隆位点，限制性内切酶
载体大小:	10410 bp
5 测序引物:	CMV-F: 5-CGCAAATGGGCGGTAGGCGTG-3
3 测序引物:	EBV-R:5-GTGGTTTGTCCAAACTCATC-3
载体标签:	/
载体抗性:	氨苄
筛选标记:	潮霉素
备注:
产品目录号:	V044-50
稳定性:	稳表达
组成型:	组成型
病毒/非病毒:	非病毒

载体质粒图谱和多克隆位点信息

载体简介

pCEP4 is an episomal mammalian expression vector that uses the cytomegalovirus (CMV) immediate early enhancer/promoter for high level transcription of recombinant genes inserted into the multiple cloning site. The Epstein-Barr Virus replication origin (oriP) and nuclear antigen (encoded by the EBNA-1 gene) is carried by this plasmid to permit extrachromosomal replication in human, primate, and canine cells. pCEP4 also carries the hygromycin B resistance gene for stable selection in transfected cells. pCEP4/CAT is provided as a positive control for the relative level of expression of recombinant proteins in a cell line of interest. It expresses the chloramphenicol acetyl transferase (CAT) protein from the CMV enhancer/promoter. Like its parent vector pCEP4, pCEP4/CAT contains the hygromycin B resistance gene for selection.

载体序列

LOCUS       pCEP4    10410 bp     DNA  circular    SYN

DEFINITION  pCEP4

ACCESSION  

KEYWORDS   

SOURCE     

  ORGANISM  other sequences; artificial sequences; vectors.

COMMENT     This file is created by Vector NTI

            http://www.biofeng.com/

COMMENT     ORIGDB|GenBank

COMMENT     VNTAUTHORNAME|biofeng.com|

FEATURES             Location/Qualifiers

     source          1..10410

                     /organism="pCEP4"

                     /mol_type="other DNA"

     misc_feature    112..134

                     /label="pGEX_3_primer"

     terminator      279..410

                     /label="SV40_PA_terminator"

     misc_feature    367..386

                     /label="EBV_rev_primer"

     promoter        complement(474..555)

                     /label="CMV_promoter"

     misc_feature    complement(474..498)

                     /label="LNCX_primer"

     misc_feature    complement(481..500)

                     /label="pCEP_fwd_primer"

     misc_feature    complement(524..544)

                     /label="CMV_fwd_primer"

     CDS             1305..2045

                     /label="ORF frame 3"

     terminator      complement(1566..1836)

                     /label="TK_PA_terminator"

     CDS             complement(1844..2869)

                     /label="ORF frame 3"

     gene            complement(1847..2854)

                     /label="hygroB"

                     /gene="hygroB"                 

     CDS             2111..3481

                     /label="ORF frame 2"                  

     promoter        2988..3136

                     /label="TATA_promoter"

     gene            3143..3481

                     /label="tet (852 - 1190)"

                     /gene="tet (852 - 1190)"

     CDS             3385..3996

                     /label="ORF frame 1"

     gene            complement(4430..5290)

                     /label="Ampicillin"

                     /gene="Ampicillin"

     CDS             complement(4430..5290)

                     /label="ORF frame 3"

     promoter        complement(5332..5360)

                     /label="AmpR_promoter"

     CDS             complement(5914..7128)

                     /label="ORF frame 1"

     CDS             5916..7841

                     /label="ORF frame 3"

     CDS             6031..7143

                     /label="ORF frame 1"

     rep_origin      8145..10315

                     /label="EBV_origin"

ORIGIN

    1 tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct

   61 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct

  121 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc

  181 agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga

  241 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg

  301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt

  361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cggccttgcc

  421 ggcctcgagc ggccgctagc aagcttgcta gcagctggta cccagcttct agagatctga

  481 cggttcacta aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt

  541 tgcgtcaacg gggcggggtt attacgacat tttggaaagt cccgttgatt ttggtgccaa

  601 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc

  661 tatccacgcc cattggtgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat

  721 acgtagatgt actgccaagt aggaaagtcc cgtaaggtca tgtactgggc ataatgccag

  781 gcgggccatt taccgtcatt gacgtcaata gggggcggac ttggcatatg atacacttga

  841 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc

  901 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg

  961 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg

 1021 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa

 1081 catggcggtc atattggaca tgagccaata taaatgtaca tattatgata tagatacaac

 1141 gtatgcaatg gccaatagcc aatattgatt tatgctatat aaccaatgac taatatggct

 1201 aattgccaat attgattcaa tgtatagatc ttccatacct accagttctg cgcctgcagc

 1261 aatgcaacaa cgttgcccgg atctgcgatg ataagctgtc aaacatgaga attggtcgac

 1321 tagcttggca cgccagaaat ccgcgcggtg gtttttgggg gtcgggggtg tttggcagcc

 1381 acagacgccc ggtgttcgtg tcgcgccagt acatgcggtc catgcccagg ccatccaaaa

 1441 accatgggtc tgtctgctca gtccagtcgt ggaccagacc ccacgcaacg cccaaaataa

 1501 taacccccac gaaccataaa ccattcccca tgggggaccc cgtccctaac ccacggggcc

 1561 agtggctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc cttcacccga

 1621 acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc aatggggtct

 1681 cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga

 1741 cccaacaccc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg

 1801 gtattgtctc cttccgtgtt tcagttagcc tcccccatct cccctattcc tttgccctcg

 1861 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc

 1921 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga

 1981 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc

 2041 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg

 2101 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg

 2161 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc

 2221 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg

 2281 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag

 2341 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg

 2401 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc

 2461 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct

 2521 ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac

 2581 cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca

 2641 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga

 2701 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga

 2761 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt

 2821 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc

 2881 cgggatctgc ggcacgctgt tgacgctgtt aagcgggtcg ctgcagggtc gctcggtgtt

 2941 cgaggccaca cgcgtcacct taatatgcga agtggacctg ggaccgcgcc gccccgactg

 3001 catctgcgtg ttcgaattcg ccaatgacaa gacgctgggc ggggtttgtg tcatcataga

 3061 actaaagaca tgcaaatata tttcttccgg ggacaccgcc agcaaacgcg agcaacgggc

 3121 cacggggatg aagcagggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc

 3181 gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat

 3241 gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca

 3301 aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc

 3361 gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct

 3421 ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg

 3481 aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa

 3541 ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc

 3601 gccatctcca gcagccgcac gcggcgcagc aaaaggccag gaaccgtaaa aaggccgcgt

 3661 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa

 3721 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct

 3781 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc

 3841 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg

 3901 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct

 3961 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag

 4021 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga

 4081 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga

 4141 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg

 4201 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag

 4261 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag

 4321 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat

 4381 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct

 4441 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac

 4501 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa

 4561 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg

 4621 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt

 4681 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca

 4741 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt

 4801 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct

 4861 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg

 4921 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg

 4981 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg

 5041 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa

 5101 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt

 5161 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt

 5221 gagcaaaaac aggaaggcaa aatgccgcaa, aaaagggaat aagggcgaca cggaaatgtt

 5281 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca

 5341 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat

 5401 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata

 5461 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt ctcatgtttg acagcttatc

 5521 atcgataagc tgatcctcac aggccgcacc cagcttttct tccgttgccc cagtagcatc

 5581 tctgtctggt gaccttgaag aggaagagga ggggtcccga gaatccccat ccctaccgtc

 5641 cagcaaaaag ggggacgagg aatttgaggc ctggcttgag gctcaggacg caaatcttga

 5701 ggatgttcag cgggagtttt ccgggctgcg agtaattggt gatgaggacg aggatggttc

 5761 ggaggatggg gaattttcag acctggatct gtctgacagc gaccatgaag gggatgaggg

 5821 tgggggggct gttggagggg gcaggagtct gcactccctg tattcactga gcgtcgtcta

 5881 ataaagatgt ctattgatct cttttagtgt gaatcatgtc tgacgagggg ccaggtacag

 5941 gacctggaaa tggcctagga gagaagggag acacatctgg accagaaggc tccggcggca

 6001 gtggacctca aagaagaggg ggtgataacc atggacgagg acggggaaga ggacgaggac

 6061 gaggaggcgg aagaccagga gccccgggcg gctcaggatc agggccaaga catagagatg

 6121 gtgtccggag accccaaaaa cgtccaagtt gcattggctg caaagggacc cacggtggaa

 6181 caggagcagg agcaggagcg ggaggggcag gagcaggagg ggcaggagca ggaggagggg

 6241 caggagcagg aggaggggca ggaggggcag gaggggcagg aggggcagga gcaggaggag

 6301 gggcaggagc aggaggaggg gcaggagggg caggaggggc aggagcagga ggaggggcag

 6361 gagcaggagg aggggcagga ggggcaggag caggaggagg ggcaggaggg gcaggagggg

 6421 caggagcagg aggaggggca ggagcaggag gaggggcagg aggggcagga gcaggaggag

 6481 gggcaggagg ggcaggaggg gcaggagcag gaggaggggc aggagcagga ggggcaggag

 6541 gggcaggagg ggcaggagca ggaggggcag gagcaggagg aggggcagga ggggcaggag

 6601 gggcaggagc aggaggggca ggagcaggag gggcaggagc aggaggggca ggagcaggag

 6661 gggcaggagg ggcaggagca ggaggggcag gaggggcagg agcaggaggg gcaggagggg

 6721 caggagcagg aggaggggca ggaggggcag gagcaggagg aggggcagga ggggcaggag

 6781 caggaggggc aggaggggca ggagcaggag gggcaggagg ggcaggagca ggaggggcag

 6841 gaggggcagg agcaggagga ggggcaggag caggaggggc aggagcagga ggtggaggcc

 6901 ggggtcgagg aggcagtgga ggccggggtc gaggaggtag tggaggccgg ggtcgaggag

 6961 gtagtggagg ccgccggggt agaggacgtg aaagagccag ggggggaagt cgtgaaagag

 7021 ccagggggag aggtcgtgga cgtggagaaa agaggcccag gagtcccagt agtcagtcat

 7081 catcatccgg gtctccaccg cgcaggcccc ctccaggtag aaggccattt ttccaccctg

 7141 taggggaagc cgattatttt gaataccacc aagaaggtgg cccagatggt gagcctgacg

 7201 tgcccccggg agcgatagag cagggccccg cagatgaccc aggagaaggc ccaagcactg

 7261 gaccccgggg tcagggtgat ggaggcaggc gcaaaaaagg agggtggttt ggaaagcatc

 7321 gtggtcaagg aggttccaac ccgaaatttg agaacattgc agaaggttta agagctctcc

 7381 tggctaggag tcacgtagaa aggactaccg acgaaggaac ttgggtcgcc ggtgtgttcg

 7441 tatatggagg tagtaagacc tccctttaca acctaaggcg aggaactgcc cttgctattc

 7501 cacaatgtcg tcttacacca ttgagtcgtc tcccctttgg aatggcccct ggacccggcc

 7561 cacaacctgg cccgctaagg gagtccattg tctgttattt catggtcttt ttacaaactc

 7621 atatatttgc tgaggttttg aaggatgcga ttaaggacct tgttatgaca aagcccgctc

 7681 ctacctgcaa tatcagggtg actgtgtgca gctttgacga tggagtagat ttgcctccct

 7741 ggtttccacc tatggtggaa ggggctgccg cggagggtga tgacggagat gacggagatg

 7801 aaggaggtga tggagatgag ggtgaggaag ggcaggagtg atgtaacttg ttaggagacg

 7861 ccctcaatcg tattaaaagc cgtgtattcc cccgcactaa agaataaatc cccagtagac

 7921 atcatgcgtg ctgttggtgt atttctggcc atctgtcttg tcaccatttt cgtcctccca

 7981 acatggggca attgggcata cccatgttgt cacgtcactc agctccgcgc tcaacacctt

 8041 ctcgcgttgg aaaacattag cgacatttac ctggtgagca atcagacatg cgacggcttt

 8101 agcctggcct ccttaaattc acctaagaat gggagcaacc agcatgcagg aaaaggacaa

 8161 gcagcgaaaa ttcacgcccc cttgggaggt ggcggcatat gcaaaggata gcactcccac

 8221 tctactactg ggtatcatat gctgactgta tatgcatgag gatagcatat gctacccgga

 8281 tacagattag gatagcatat actacccaga tatagattag gatagcatat gctacccaga

 8341 tatagattag gatagcctat gctacccaga tataaattag gatagcatat actacccaga

 8401 tatagattag gatagcatat gctacccaga tatagattag gatagcctat gctacccaga

 8461 tatagattag gatagcatat gctacccaga tatagattag gatagcatat gctatccaga

 8521 tatttgggta gtatatgcta cccagatata aattaggata gcatatacta ccctaatctc

 8581 tattaggata gcatatgcta cccggataca gattaggata gcatatacta cccagatata

 8641 gattaggata gcatatgcta cccagatata gattaggata gcctatgcta cccagatata

 8701 aattaggata gcatatacta cccagatata gattaggata gcatatgcta cccagatata

 8761 gattaggata gcctatgcta cccagatata gattaggata gcatatgcta tccagatatt

 8821 tgggtagtat atgctaccca tggcaacatt agcccaccgt gctctcagcg acctcgtgaa

 8881 tatgaggacc aacaaccctg tgcttggcgc tcaggcgcaa gtgtgtgtaa tttgtcctcc

 8941 agatcgcagc aatcgcgccc ctatcttggc ccgcccacct acttatgcag gtattccccg

 9001 gggtgccatt agtggttttg tgggcaagtg gtttgaccgc agtggttagc ggggttacaa

 9061 tcagccaagt tattacaccc ttattttaca gtccaaaacc gcagggcggc gtgtgggggc

 9121 tgacgcgtgc ccccactcca caatttcaaa aaaaagagtg gccacttgtc tttgtttatg

 9181 ggccccattg gcgtggagcc ccgtttaatt ttcgggggtg ttagagacaa ccagtggagt

 9241 ccgctgctgt cggcgtccac tctctttccc cttgttacaa atagagtgta acaacatggt

 9301 tcacctgtct tggtccctgc ctgggacaca tcttaataac cccagtatca tattgcacta

 9361 ggattatgtg ttgcccatag ccataaattc gtgtgagatg gacatccagt ctttacggct

 9421 tgtccccacc ccatggattt ctattgttaa agatattcag aatgtttcat tcctacacta

 9481 gtatttattg cccaaggggt ttgtgagggt tatattggtg tcatagcaca atgccaccac

 9541 tgaacccccc gtccaaattt tattctgggg gcgtcacctg aaaccttgtt ttcgagcacc

 9601 tcacatacac cttactgttc acaactcagc agttattcta ttagctaaac gaaggagaat

 9661 gaagaagcag gcgaagattc aggagagttc actgcccgct ccttgatctt cagccactgc

 9721 ccttgtgact aaaatggttc actaccctcg tggaatcctg accccatgta aataaaaccg

 9781 tgacagctca tggggtggga gatatcgctg ttccttagga cccttttact aaccctaatt

 9841 cgatagcata tgcttcccgt tgggtaacat atgctattga attagggtta gtctggatag

 9901 tatatactac tacccgggaa gcatatgcta cccgtttagg gttaacaagg gggccttata

 9961 aacactattg ctaatgccct cttgagggtc cgcttatcgg tagctacaca ggcccctctg

10021 attgacgttg gtgtagcctc ccgtagtctt cctgggcccc tgggaggtac atgtccccca

10081 gcattggtgt aagagcttca gccaagagtt acacataaag gcaatgttgt gttgcagtcc

10141 acagcatgca aagtctgctc caggatgaaa gccactcagt gttggcaaat gtgcacatcc

10201 atttataagg atgtcaacta cagtcagaga acccctttgt gtttggtccc cccccgtgtc

10261 acatgtggaa cagggcccag ttggcaagtt gtaccaacca actgaaggga ttacatgcac

10321 tgccccgaat acaaaacaaa agcgctcctc gtaccagcga agaaggggca gagatgccgt

10381 agtcaggttt agttcgtccg gcggcggggc

//

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