Recombinant Mouse Leukemia inhibitory factor /mouse LIF

 

Recombinant(E.coli)

Lyophilizate,sterile-filtered

 

Cat.No.        RP1001-2UG                2µg                 store at -20℃

Cat.No.        RP1001-10UG              10µg                 store at -20℃

 

Synonyms : CDF, HILDA, D-FACTOR, Differentiation- stimulating factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, Leukemia inhibitory factor, LIF, DIA.

 

Description:  Leukemia Inhibitory Factor (LIF) Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 181 amino acids and having a molecular mass of 20 kDa. The Leukemia Inhibitory Factor (LIF) is purified by proprietary chromatographic techniques.

 

Source:        Escherichia Coli.

 

Purity:         Greater than 90.0% as determined by SDS-PAGE.

Endotoxin Level : Endotoxin level is less than 0.1 ng per μg (1EU/μg) as determined  by the LAL method.

Biological Activity : Activity of mouse LIF was determined by its ability to induce differentiation of murine M1myeloid leukemic cells. Min. detectable conc. of murine LIF in this specific bioassay was found to be 0.5 ng/mL. The specific activity is 100MIU/mg, where 50U is defined as the amount of murine LIF required to induce differentiation in 50% of the M1 colonies per ml of agar culture.

Amino acid sequence:

GSPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPFPNNVEKLCAPNMTDFPSFHGNGTEKTKLVELYRMVAYLSASLTNITRDQKVLNPTAVSLQVKLNATIDVMRGLLSNVLCRLCNKYRVGHVDVPPVPDHSDKEAFQRKKLGCQLLGTYKQVISVVVQAF

Formulation: mouse LIF was lyophilized from sterile PBS , pH 7.4

Solubility: It is recommended to reconstitute the lyophilized Leukemia Inhibitory Factor (LIF) in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized Leukemia Inhibitory Factor (LIF) although stable at room temperature for 3 weeks, should be stored desiccated below -20°C. Upon reconstitution FGFb should be stored at 4°C between 2-7 days and for future use below -20°C.
          For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. Recommend to aliquot the protein into smaller quantities for optimal storage.

Usage: Follow the instructions on the vial. Centrifuge the vial at 4℃ before opening to recover the entire contents.

The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 

Background

Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence.

 

 

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