C-Raf原癌基因丝苏氨酸蛋白激酶(CRAF)重组蛋白

YB232Hu012
Recombinant C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
C-Raf原癌基因丝苏氨酸蛋白激酶(CRAF)重组蛋白[ PROPERTIES ]
Residues: Trp342~Ser619
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P04049
Host: E. coli
Subcellular Location: Cytoplasm. Cell
membrane. Mitochondrion. Nucleus.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
C-Raf原癌基因丝苏氨酸蛋白激酶(CRAF)重组蛋白150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 9.2
Predicted Molecular Mass: 35.6kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
C-Raf原癌基因丝苏氨酸蛋白激酶(CRAF)重组蛋白that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
WEIEASEVM LSTRIGSGSF GTVYKGKWHG DVAVKILKVV DPTPEQFQAF RNEVAVLRKT
RHVNILLFMG YMTKDNLAIV TQWCEGSSLY KHLHVQETKF QMFQLIDIAR QTAQGMDYLH
AKNIIHRDMK SNNIFLHEGL TVKIGDFGLA TVKSRWSGSQ QVEQPTGSVL WMAPEVIRMQ
DNNPFSFQSD VYSYGIVLYE LMTGELPYSH INNRDQIIFM VGRGYASPDL SKLYKNCPKA
MKRLVADCVK KVKEERPLFP QILSSIELLQ HSLPKINRS
[ REFERENCES ]
1. Bonner T.I., et al. (1986) Nucleic Acids Res. 14:1009-1015. 2. Bonner T.I., et al. (1985) Mol. Cell. Biol. 5:1400-1407. 3. Andreu-Perez P., et al. (2011) Sci. Signal. 4:RA58-RA58. 4. Dozier C., et al. (1991) Oncogene 6:1307-1311.