GAPDH antibody Application Information: GAPDH antibody | Product Name | GAPDH antibody | | Applications | ICC/IF, IHC-P, IP, WB, IHC-R, IB | | Application Note | | Suggested dilution | | ICC/IF | 1:100-1:1000* | | IHC (Formalin-fixed paraffin-embedded sections) | 1:100-1:1000* | | Immunoprecipitation | 1:100-1:500* | | Western blot | 1:1000-1:100000* | | IHC (Resin sections) | Assay-dependent dilution | | Immunoblotting | Assay-dependent dilution |
| | Predicted Target Size | 36 kDa (note) | | Positive Controls | 293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , E.coli M15 , *SH-SY5Y , *A549 , *U2OS , *H9C2 , *A7R5 , *mouse skin tissue | | Form Supplied | Liquid | | Concentration | 1 mg/ml (Please refer to the vial label for the specific concentration) | | Purification | Purified by antigen-affinity chromatography. | Specifications: GAPDH antibody | Full Name | glyceraldehyde-3-phosphate dehydrogenase | | Product Description | Rabbit Polyclonal antibody to GAPDH (glyceraldehyde-3-phosphate dehydrogenase) | | Background | The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq] | | Synonyms | G3PD antibody, GAPD antibody, MGC88685 antibody, GAPDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, aging-associated gene 9 protein antibody, glyceraldehyde 3-phosphate dehydrogenase antibody, peptidyl-cysteine S-nitrosylase GAPDH antibody | | Cellular Localization | Cytoplasm , perinuclear region , Membrane | | Host | Rabbit | | Clonality | Polyclonal | | Isotype | IgG | | Immunogen | Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary. | | Antigen Species | Human | | Species Reactivity | Human, Mouse, Dog, Drosophila, Rat, E. coli | | Predicted Cross Reactivity species | Zebrafish, Xenopus laevis, Xenopus Tropicalis, Cat, Pig, Rabbit, Chicken, Sheep, Rhesus Monkey, Bovine, Guinea pig | | Predict Reactivity Note | Zebrafish (86%), Xenopus laevis (85%), Xenopus Tropicalis (85%), Cat (96%), Pig (95%), Rabbit (94%), Chicken (92%), Sheep (94%), Rhesus Monkey (99%), Bovine (94%), Guinea pig (94%) | | Conjugation | Unconjugated | Storage Conditions: GAPDH antibody | Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. | | Storage Instruction | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. | | Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. | |