10X Tris-Glycine-SDS Transfer buffer
英文名称： 10X Tris-Glycine-SDS Transfer buffer
型号:null    抗体货号： MP041-100ML
价格:请致电：010-57128832,18610462672
品牌: 国产

 10X Tris-Glycine-SDS Transfer buffer

产品简介

本品组分浓度为480mM Tris，390mM glycine，0.37% SDS(W/V)。加入约600 mL的去离子水，充分搅拌溶解后，加去离子水定容至800 mL，经0.22um滤膜过滤后，再加入200 mL甲醇，配制而成。本品稀释10倍为工作液。

保存条件

室温保存。

, IHC-R, IB

Application Note

              Suggested dilution

ICC/IF

1:100-1:1000*

IHC (Formalin-fixed paraffin-embedded sections)

1:100-1:1000*

Immunoprecipitation

1:100-1:500*

Western blot

1:1000-1:100000*

IHC (Resin sections)

Assay-dependent dilution

Immunoblotting

Assay-dependent dilution

 

 

Predicted Target Size

36 kDa (note)

Positive Controls

293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , E.coli M15 , *SH-SY5Y , *A549 , *U2OS , *H9C2 , *A7R5 , *mouse skin tissue

Form Supplied

Liquid

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Purified by antigen-affinity chromatography.

 

 

 

Specifications: GAPDH antibody

 

Full Name

glyceraldehyde-3-phosphate dehydrogenase

Product Description

Rabbit Polyclonal antibody to GAPDH (glyceraldehyde-3-phosphate dehydrogenase)

Background

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]

Synonyms

G3PD antibody, GAPD antibody, MGC88685 antibody, GAPDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, aging-associated gene 9 protein antibody, glyceraldehyde 3-phosphate dehydrogenase antibody, peptidyl-cysteine S-nitrosylase GAPDH antibody

Cellular Localization

Cytoplasm , perinuclear region , Membrane

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.

Antigen Species

Human

Species Reactivity

Human, Mouse, Dog, Drosophila, Rat, E. coli

Predicted Cross Reactivity species 

Zebrafish, Xenopus laevis, Xenopus Tropicalis, Cat, Pig, Rabbit, Chicken, Sheep, Rhesus Monkey, Bovine, Guinea pig

Predict Reactivity Note

Zebrafish (86%), Xenopus laevis (85%), Xenopus Tropicalis (85%), Cat (96%), Pig (95%), Rabbit (94%), Chicken (92%), Sheep (94%), Rhesus Monkey (99%), Bovine (94%), Guinea pig (94%)

Conjugation

Unconjugated

 

 

Storage Conditions: GAPDH antibody

 

Storage Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage Instruction

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Notes

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.