崩溃酶 Driselase

Descriptions：

一种粗制酶,主要含纤维素酶和果胶酶,还混有蛋白酶和核酸酶.加速培养细胞核根尖细胞离解。崩溃酶(Driselase)是可以水解细胞壁(主要是真菌细胞壁)的多种酶的混合物,制备原生质体很有用. 来自sigma公司说明：Crude powder containing laminarinase, xylanase and cellulase.

Properties：

Product Name： Driselase from Basidiomycetes sp.,

powder, Protein ≥10 % by biuret

Product Number:D9515

Product Brand:Sigma

CAS Number:85186-71-6

Storage Temp:-20°C

应用：

For the preparation of protoplasts1,2; Analysis of pre-mRNA processing in transfected plant protoplasts3

配制方法：:

Place 0.2 g driselase in a conical flask, dissolve in 10 mL 4 M mannitol and adjust the pH with 0.1N HCl to 5.2. Suspend 1 g of cultured cells in the solution. When the enzyme is allowed to react with the cells for 1-2 hours while gently shaking the flask in a water bath at 30°C, most of the cells used as materials lose the cell walls and become spherical protoplasts.

Unit Definition  

单位定义：

1 U corresponds to the amount of enzyme which liberates 1 μmol glucose from carboxymethylcellulose per minute at pH 5.0 and 37°C; further enzyme activities: pectinase, xylanase, ***ase, laminarinase, amylase, protease, macerating and lytic activity; commonly used concentration: 1.0-2.5 %

产品的技术指标：

D9515-Enzyme Assay (19kb)    MSDS     Specification Sheet  Carbohydrate Analysis bf8

订货信息：

产地：Sigma    货号：D9515    

现货供应：810元/1g   促销价：680元/1g    110元/100mg  Descriptions