海藻糖检测试剂盒 英文名称: Trehalose Assay Kit |
型号:null 产品货号: K-TREH |
价格:请致电:010-57128832,18610462672 |
品牌: 爱尔兰 |
品牌: Megazyme 产品货号:K-TREH 品牌:Megazyme 产品品名:海藻糖检测试剂盒 英文品名:Trehalose Assay Kit 规格型号:100 assays (manual) / 1000 assays (microplate) / 1100 assays (auto-analyser) A simple method for the rapid and reliable measurement of trehalose infoods, beverages and other materials. Suitable for manual, auto-analyser and microplate formats. INTRODUCTION: Trehalose is a naturally occurring disaccharide containing two glucose molecules bound in an α,α-1,1 linkage. This structure results in a chemically stable, non-reducing sugar with many important functional characteristics. Trehalose is commonly found in nature, provides a source of energy, and has been shown to be a primary factor in stabilising organisms during times of freezing, drying and heating. Trehalose is consumed as part of a normal diet in mushrooms, honey, lobster, shrimp and foods produced using baker’s and brewer’s yeast. Trehalose has been determined to be generally recognised as safe (GRAS) as a multipurpose ingredient for all uses in food, including as a sweetener, stabiliser, thickener and flavour enhancer, in general accordance with current good manufacturing practices. It has a uniquely high glass transition temperature for a disaccharide, is nonbrowning, and will not hydrolyse at low pH or high temperatures. This results in more stable colours and flavours in foods to which it is added. Trehalose is 45% as sweet as sucrose when compared in a 10% (w/v) solution and is stable at up to 94% relative humidity. The low hygroscopic nature of trehalose dihydrate results in a freeflowing, stable dry product. As a non-reducing sugar it does not react with amino acids or proteins to initiate Maillard browning. KITS: Kits suitable for performing 100 assays in manual format (or 1100 assays in auto-analyser format or 1000 assays in microplate format) are available from Megazyme. The kits contain the full assay method plus: Bottle 1: Buffer (25 mL, pH 7.0) plus magnesium chloride and sodium azide (0.02% w/v) as a preservative. Stable for > 2 years at 4°C. Bottle 2: NADP+ plus ATP. Stable for > 5 years at -20°C. Bottle 3: Hexokinase plus glucose-6-phosphate dehydrogenase suspension, 2.25 mL. Stable for > 2 years at 4°C. Bottle 4: Trehalase suspension (2.25 mL). Stable for > 2 years at 4°C. Bottle 5: D-Glucose standard solution (5 mL, 0.4 mg/mL) in 0.2% (w/v) benzoic acid. Stable for > 4 years at room temperature. Bottle 6: Trehalose dihydrate control (~ 2 g). Stable for > 4 years at room temperature. 温馨提示:需要详细的产品使用说明书,请联系甄准生物客服人员,我们将竭诚为您服务。 甄准,甄心倾听您每一个标准! |