L-阿拉伯糖D-半乳糖检测试剂盒 英文名称: :L-Arabinose / D-Galactose Assay Kit |
型号:null 产品货号: :L-Arabinose / D-Galactose Assay Kit |
价格:请致电:010-57128832,18610462672 |
品牌: 爱尔兰 |
品牌: Megazyme 产品货号:K-ARGA 品牌:Megazyme 产品品名:L-阿拉伯糖D-半乳糖检测试剂盒 英文品名:L-Arabinose / D-Galactose Assay Kit 规格型号:115 assays (manual) / 1150 assays (microplate) / 1150 assays (auto-analyser) A simple, reliable and accurate UV method for the measurement ofL-arabinose and/or D-galactose in various materials including foods,feeds, beverage and plant products. INTRODUCTION: Enzymic kits for the determination of L-arabinose and D-galactose are very slow. This is due to the low rate of natural chemical “mutarotation” between the α- and β-anomeric forms of these monosaccharides. Only the β-form is recognised by β-galactose dehydrogenase. In incubations containing NAD+, L-arabinose or D-galactose and β-galactose dehydrogenase, there is a very rapid initial increase in absorbance due to the consumption of β -L-arabinose or β-D-galactose, and this is followed by a very slow approach to the end-point. This very slow approach results from the very low rate of chemical “mutarotation” of the α-anomeric conformation of these monosaccharides to the respective β-anomer. Using technology developed by Megazyme (patent pending), a galactose mutarotase has now been incorporated into the assay format to rapidly catalyse this rate-limiting mutarotation step. The result is very rapid analysis times of approximately 6 min for D-galactose and approximately 12 min for L-arabinose (Figure 1). KITS: Kits suitable for performing 115 assays in manual format (or 1150 assays in microplate or auto-analyser formats) are available from Megazyme. The kits contain the full assay method plus: Bottle 1: Buffer (25 mL, pH 8.6) plus sodium azide (0.02% w/v) as a preservative. Stable for > 2 years at 4°C. Bottle 2: NAD+. Lyophilised powder. Stable for > 5 years at -20°C. Bottle 3: β-Galactose dehydrogenase plus galactose mutarotase suspension, 2.4 mL. Stable for > 2 years at 4°C. Bottle 4: L-Arabinose standard solution (5 mL, 0.4 mg/mL in 0.02% w/v sodium azide). Stable for > 2 years at 4°C. Bottle 5: D-Galactose standard solution (5 mL, 0.4 mg/mL in 0.02% w/v sodium azide). Stable for > 2 years at 4°C. 温馨提示:需要详细的产品使用说明书,请联系甄准生物客服人员,我们将竭诚为您服务。 甄准,甄心倾听您每一个标准! |