亚硫酸盐总量检测试剂盒 Total Sulphite Assay Kit 产品货号:K-TSULPH 英文名称: Total Sulphite Assay Kit |
型号:null 产品货号: K-TSULPH |
价格:请致电:010-57128832,18610462672 |
品牌: 爱尔兰 |
品牌: Megazyme 产品货号:K-TSULPH 品牌:Megazyme 产品品名:亚硫酸盐总量检测试剂盒 英文品名:Total Sulphite Assay Kit 规格型号:80 assays (manual) / 800 assays (microplate) / 800 assays (auto-analyser) AOAC Method 996.11 AACC Method 76-13.01 RACI Standard Method for themeasurement of total starch in cereal flours and food products. AACC andAOAC approved method. This kit now contains an improved α-amylase thatallows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0). INTRODUCTION: Sulphur dioxide (SO2) is used widely as an additive in various forms, most commonly as sulphites (or sulfites), in the wine, beverage and food industries where it acts primarily as an antimicrobial and antioxidant preservative. During wine making, sulphites are used as an essential additive, usually The specific formation of amylopectin complexes with the lectinconcanavalin A (Con A) offers an alternative approach to amylosemeasurement in starches, that is not subject to theseuncertainties12,13. Under defined conditions of pH, temperature andionic strength, Con A specifically complexes branched polysaccharidesbased on α-D-glucopyranosyl or α-D-mannopyranosyl units at multiplenon-reducing end-groups with the formation of a precipitate. Thus, Con Aeffectively complexes the amylopectin component of starch but not theprimarily linear amylose component. The procedure described in this booklet13 is a modification of a Con Amethod developed by Yun and Matheson (1990)13. It uses an ethanolpretreatment step to remove lipids prior to analysis [modified fromMorrison and Laignelet (1983)7]. KITS: Kits suitable for performing 100 assays are available from Megazyme.The kits contain the full assay method plus: Bottle 1: Freeze dried Con A. Stable for > 5 years at -20°C. Bottle 2: Amyloglucosidase [200 U on p-nitrophenyl β-maltoside (i.e. 3300 U on starch at pH 4.5 at 40°C)] plus fungalα-amylase (500 U on Ceralpha Reagent at pH 5.0 and 40°C), 2 mL. Stable for > 5 years at 4°C. Bottle 3: GOPOD Reagent Buffer. Buffer (48 mL, pH 7.4), p-hydroxybenzoic acid and sodium azide (0.04% w/v). Stable for > 4 years at 4°C. Bottle 4: GOPOD Reagent Enzymes. Glucose oxidase plus peroxidase and4-aminoantipyrine. Freeze- dried powder. Stable for > 5 years at -20°C. Bottle 5: D-Glucose standard solution (5 mL, 1.0 mg/mL) in 0.2% (w/v)benzoic acid. Stable for > 5 years at room temperature. Bottle 6: Starch reference sample (with specified content of amylose). Contains sodium azide (0.05 % w/v) as a preservative. Stable for > 18 months at room temperature. Bottle 3: Sulphite Standard Sodium Sulphite (5 g). Stable for > 5 years at room temperature. 温馨提示:需要详细的产品使用说明书,请联系甄准生物客服人员,我们将竭诚为您服务。 甄准,甄心倾听您每一个标准! |