28-9546-56
            
PGEX-1LT ECOR I/BAP
        
        
            
11-0033-99
            
HIS GRAVITRAP
        
        
            
11-0034-00
            
HIS BUFFER KIT
        
        
            
27-4710-01
            
ANTI-HIS ANTIBODY
        
        
            
28-4009-89
            
HIS MULTITRAP HP
        
        
            
28-4009-90
            
HIS MULTITRAP FF
        
        
            
28-4013-53
            
HIS SPINTRAP
        
        
            
28-4039-43
            
COLLECTION PLATE
        
        
            
28-9321-71
            
His SpinTrap Kit
        
        
            
27-1583-01
            
LIGHT PRIMER MIX
        
        
            
27-1586-01
            
HEAVY PRIMER MIX
        
        
            
27-1588-01
            
LINKER-PRIMER MIX
        
        
            
27-1589-01
            
RS PRIMER MIX
        
        
            
27-9420-01
            
ANTI-M13 MONOCLONAL ANTIBODY
        
        
            
27-9421-01
            
HRP/ANTI-M13 MONOCLNL CONJUG.
        
    
Anti-His Antibody
    
For the immunodetection of histidine-tagged recombinant proteins in Western blots and ELISAs.
    
Produced from a monoclonal cell line cultivated in mice and provided as clarified ascites fluid.
    
Functionally tested for detecting a test histidine-tagged recombinant protein in Western blots and ELISAs.
    
Provides sufficient monoclonal antibody to perform 25 Western blots, each containing 20 ml antibody solution
Anti-His Antibody
Technical Information

 
Anti-His Antibody
 
Anti-His Antibody is an IgG2 subclass of monoclonal antibody directed against six histidine residues, which are commonly used as an affinity tag for recombinant fusion proteins. The antibody is unconjugated to offer the flexibility of detection with a secondary antibody conjugated with either horseradish peroxidase or alkaline phosphatase. The antibody provides high sensitivity with low background. Anti-His Antibody is quality tested to detect a GST-(histidine)6-tagged protein at levels of »50 ng in ELISA and 100 ng in Western blots.
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