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Figure 1Rediject Coelenterazine has been optimized for in vivo use with IVIS imaging systems. (A) 5 pmol BRET Qdot 700* were injected intravenously in a nu/nu mouse. 10 minutes post BRET Qdot injection 15 μg/mouse Coelenterazine h was injected intravenously. Chart shows peak signal was observed right after injection at ~ 22 seconds followed by rapid clearance. Images were taken with IVIS kinectic. (B) 1,2 and 4 pmol BRET Qdot 655 were injected subcutaneously in a nu/nu mouse. 10 minutes post BRET Qdot injection 10 μg/mouse Coelenterazine was injected intraperitoneally. Chart shows that peak signal was reached around 27 minutes post i.p. injection of Coelenterazine.