usa 产品商标: peprotechBrand,null 抗体货号： 500-P07Item#, Anti-Human IL-17 (IL-17A)<br>英文名称： Anti-Human IL-17 (IL-17A)

产品编号:

500-P07

来源:

Polyclonal Rabbit

制备:

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-17A. Anti-Human IL-17A specific antibody was purified by affinity chromatography employing immobilized hIL-17 matrix.

免疫原:

E.coli derived Recombinant Human IL-17 (IL-17A) (PeproTech catalog #200-17)

应用:

ELISA

Sandwich
To detect hIL-17A by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-17A (500-P07Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17A.

Western Blot

To detect hIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Neutralization

To yield one-half maximal inhibition [ND₅₀] of the biological activity of hIL-17A (50.0 ng/ml), a concentration of 0.9-1.3 μg/ml of this antibody is required.

Immunohistochemistry

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.25 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.