usa 产品商标: peprotechBrand,null 抗体货号： 500-P118Item#, Anti-Murine RANTES<br>英文名称： Anti-Murine RANTES

产品编号:

500-P118

来源:

Polyclonal Rabbit

制备:

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mRANTES. Anti-Murine RANTES specific antibody was purified by affinity chromatography employing immobilized mRANTES matrix.

免疫原:

E.coli derived Recombinant Murine RANTES (CCL5) (PeproTech catalog #250-07)

应用:

ELISA

Sandwich
To detect mRANTES by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine RANTES (500-P118Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mRANTES.

Western Blot

To detect mRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mRANTES is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Neutralization

To yield one-half maximal inhibition [ND50] of the biological activity of mRANTES (100.00 ng/ml), a concentration of 0.8-1.2 µg/ml of this antibody is required.

交叉反应:

Rat, Mouse