NucleoBond® Xtra Midi / MaxiTransfection-grade plasmid DNA60% time-saving, 100% increased yieldsFeatures The new generation of ultra-fast and high-yield, transfection-grade plasmid DNA purification at Midiprep and Maxiprep scaleNucleoBond Xtra Midi Column• Optimized column design: plasmid Midiprep in approximately 30 min, plasmid Maxiprep in 35 min• Column filter included: high filter flow rates, parallel lysate clearing and loading onto the column• Improved silica material: typical yields of 250 μg (Midi) and 1 000 μg (Maxi)• Transfection-grade plasmid DNA due to patented anion-exchange technology• NucleoBond® Xtra Plus kits contain NucleoBond® Finalizers to speed up DNA precipitation NucleoBond® Xtra Midi NucleoBond® Xtra MaxiTechnology Anion-exchange chromatography Anion-exchange chromatographyFormat Midi gravity flow columns Maxi gravity flow columnsLysate clarification Column filters Column filtersSample material < 200 mL (high copy)< 400 mL (low copy) < 600 mL (high copy)< 1200 mL (low copy)Vector size< 300 kbp < 300 kbpTypical yield 250 μg 1000 μgA260/A280 1.80–1.95 1.80–1.95Isopropanol precipitation Xtra MidiCentrifugationXtra Midi PlusNucleoBond® FinalizerXtra MaxiCentrifugationXtra Maxi PlusNucleoBond® Finalizer LargePreparation time 70 min/4 preps 30 min/4 preps 80 min/4 preps 35 min/4 preps Applications • High-copy plasmids from E. coli • Low-copy plasmids from E. coli* * Increased buffer volumes required. See "Ordering information"