谷胱甘肽过氧化物酶分析试剂盒 英文名称: GPx | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
型号:null 产品货号: 8238 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
价格:请致电:010-57128832,18610462672 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
品牌: 美国sciencell | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
北京裕恒丰科技有限公司,已成功代理美国Sciencell公司细胞生物学产品十余年。多年来,裕恒丰一直秉承信誉至上的原则,在点滴中慢慢地提升企业信誉,树立企业形象,我们始终以热心的服务和专业的技术支持,为广大科研工作者提供了优质的细胞及分子生物学产品。在未来的发展中,我们将继续努力打造企业品牌,北京裕恒丰不仅希望您能聆听到我们的心声,更期待您能亲自体验我们的服务,北京裕恒丰欢迎您的光临。 温馨提示:ScienCell产品仅用于科研,不能用于人或动物以及体外诊断应用。如预购买产品请登陆ScienCell唯一官网(www.sciencellonline.com)寻找正规代理商,以确保您的实验顺利进行。 谷胱甘肽过氧化物酶分析试剂盒 货号8238 Glutathione peroxidase (GPx) is an antioxidant enzyme located in cytoplasma and mitochondria. It catalyzes the reduction of hydrogen peroxide (H2O2) and a variety of organic hydroperoxides (R-O-O-H) to water and the corresponding stable alcohols (R-OH) using glutathione as the reducing reagent, thus inhibiting the formation of free radicals and protecting the cells from oxidative damage. The ScienCell GPx Assay provides an indirect method to quantify the activity of total glutathione peroxidase. In this assay, oxidized glutathione, produced upon reduction of peroxide substrate by glutathione peroxidase, is then recycled back to glutathione using glutathione reductase and NADPH. The oxidation of NADPH to NADP+ is monitored by a decrease in the absorbance at 340 nm. Since all other reagents are provided in excess, the GPx activity is rate-limiting. The rate of decrease in A340nm is directly proportional to the GPx activity. In this assay, cumene hydroperoxide is used as the peroxide substrate, so the total GPx (selenium and non-selenium containing) activity in a variety of samples can be measured.
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