产品名称：人视网膜色素上皮细胞
英文名称：Human Retinal Pigment Epithelial Cells
货号：6540
品牌：ScienCell
产地：美国
规格：5 x10^5，1ml
价格：$676


产品说明
     视网膜是位于眼睛后部的多层结构。它包括感光细胞和视网膜色素上皮细胞（RPEpiC）。RPEpiC位于神经视网膜和脉络膜之间，形成视网膜外屏障，控制着视网膜下腔的化学构成。人们发现RPEpiC内有许多特定偏振分布的细胞内蛋白质。与其他上皮细胞通常顶生面没有基质相比，RPEpiC的顶生面胞膜是与细胞外基质相接触的。RPEpiC在调节相邻的感光膜周转、维生素A的代谢、保护细胞内黑色素所致的视网膜光损害中起关键作用。RPEpiC与很多视网膜疾病的发病相关，如增殖性视网膜病变和年龄相关性黄斑变性。
       Sciencell研究实验室的HRPEpiC是从人视网膜分离得到的，原代冻存，冰冻运输。每管细胞密度超过5×10^5/ml。细胞特性经cytokeratine -18, -19和纤维粘连蛋白免疫荧光鉴定。本细胞经检测不含HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌。如采用ScienCell 实验室特制的培养基，可保证此细胞10倍增增殖。

推荐培养基
上皮细胞培养基（货号：4101 ）
 
使用说明
人视网膜色素上皮细胞 仅用于科研。不能用于人或动物和体外诊断应用。
 
储存
接收到冻存细胞时请立即从干冰转入液氮中保存。
 
运输
干冰运输。
 
文献
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2.) Miyamoto N, Izumi H, Miyamoto R, Bin H, Kondo H, Tawara A, Sasaguri Y, Kohno K. (2011) "Transcriptional Regulation of Activating Transcription Factor 4 under Oxidative Stress in Retinal Pigment Epithelial ARPE-19/HPV-16 Cells." Invest Ophthalmol Vis Sci. 52: 1226-34.

3.) Narayan S, Prasanna G, Tchedre K, Krishnamoorthy R, Yorio T. (2010) "Thrombin-induced endothelin-1 synthesis and secretion in retinal pigment epithelial cells is rho kinase dependent." J Ocul Pharmacol Ther. 26: 389-97.

4.) Bertram KM, Baglole CJ, Phipps RP, Libby RT. (2009) "Molecular regulation of cigarette smoke induced-oxidative stress in human retinal pigment epithelial cells: implications for age-related macular degeneration." Am J Physiol Cell Physiol. 297: C1200-10.

5.) Mannermaa E, Vellonen KS, Ryh?nen T, Kokkonen K, Ranta VP, Kaarniranta K, Urtti A. (2009) "Efflux protein expression in human retinal pigment epithelium cell lines." Pharm Res. 26: 1785-91.

6.) Cheong HI, Johnson J, Cormier M, Hosseini K. (2008) "In vitro cytotoxicity of eight beta-blockers in human corneal epithelial and retinal pigment epithelial cell lines: comparison with epidermal keratinocytes and dermal fibroblasts." Toxicol In Vitro. 22: 1070-6.

7.) Swaney JS, Moreno KM, Gentile AM, Sabbadini RA, Stoller GL. (2008) "Sphingosine-1-phosphate (S1P) is a novel fibrotic mediator in the eye." Exp Eye Res. 87: 367-75.

8.) Hunt DW, Boivin WA, Fairley LA, Jovanovic MM, King DE, Salmon RA, Utting OB. (2006) "Ultraviolet B light stimulates interleukin-20 expression by human epithelial keratinocytes." Photochem Photobiol. 82: 1292-300.