猪角膜上皮细胞 英文名称: PCEpiC |
型号:null 产品货号: P6510 |
价格:请致电:010-57128832,18610462672 |
品牌: 美国sciencell |
北京裕恒丰科技有限公司,已成功代理美国Sciencell公司细胞生物学产品十余年。多年来,裕恒丰一直秉承信誉至上的原则,在点滴中慢慢地提升企业信誉,树立企业形象,我们始终以热心的服务和专业的技术支持,为广大科研工作者提供了优质的细胞及分子生物学产品。在未来的发展中,我们将继续努力打造企业品牌,北京裕恒丰不仅希望您能聆听到我们的心声,更期待您能亲自体验我们的服务,北京裕恒丰欢迎您的光临。 温馨提示:ScienCell从不提供永生化细胞,所有细胞均为正常原代细胞。ScienCell在中国地区不销售分装后的细胞。如预购买产品请登陆ScienCell唯一官网(www.sciencellonline.com)寻找正规代理商,以确保您的实验顺利进行。 猪角膜上皮细胞 货号P6510 细胞来源 The cornea is a unique tissue due to its transparency and avascularity. The cornea consists of three distinct cell layers: the outer epithelium, the central stroma composed of interconnected keratocytes, and the inner endothelium. The corneal epithelium plays a role in the innate immune response by sensing the presence of pathogens and providing signals to activate the corneal defense system [1]. Corneal epithelial cells also express a high level of aldehyde dehydrogenase to protect them against UV- and 4-hydroxynonenal-induced cellular damage [2]. The renewal of corneal epithelium is regulated by a host of cytokines, including epidermal growth factor, which activates their cognate receptors in the deeper layers of the epithelium [3]. Cultured corneal epithelial cells can be an alternative to animal testing in toxicology studies. They are also suitable for understanding the highly integrated balance between the processes of corneal epithelial proliferation, differentiation, and cell death. PCEpiC from ScienCell Research Laboratories are isolated from porcine cornea. PCEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. PCEpiC are characterized by their cobble stone morphology in serum-free culture and immunofluorescence with antibodies specific to CK-18 and -19. PCEpiC are negative for mycoplasma, bacteria, yeast, and fungi. PCEpiC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories. 推荐培养基 It is recommended to use Corneal Epithelial Cell Medium-C (CEpiCM-C, Cat. #6511) for culturing PCEpiC in vitro. 参考文献 [1] Zhang J, Wu XY, Yu FS. (2005) “Inflammatory response of corneal epithelial cells to pseudomonas aeruginosa infection.” Curr Eye Res. 30: 527-34. [2] Pappa A, Brown D, Koutalos Y, Degregori J, White C, Vasiliou V. (2005) “Human aldehyde dehydrogenase 3A1 inhibits proliferation and promotes survival of human corneal epithelial cells.” J Biol Chem. 280: 27998-8006. [3] Lu L, Reinach PS, Kao WW. (2001) “Corneal epithelial wound healing.” Exp Biol Med (Maywood). 226: 653-64. |