星形胶质细胞转染试剂盒 英文名称: AstroFectagen |
型号:null 产品货号: 0943 |
价格:请致电:010-57128832,18610462672 |
品牌: 美国sciencell |
北京裕恒丰科技有限公司,已成功代理美国Sciencell公司细胞生物学产品十余年。多年来,裕恒丰一直秉承信誉至上的原则,在点滴中慢慢地提升企业信誉,树立企业形象,我们始终以热心的服务和专业的技术支持,为广大科研工作者提供了优质的细胞及分子生物学产品。在未来的发展中,我们将继续努力打造企业品牌,北京裕恒丰不仅希望您能聆听到我们的心声,更期待您能亲自体验我们的服务,北京裕恒丰欢迎您的光临。 温馨提示:ScienCell产品仅用于科研,不能用于人或动物以及体外诊断应用。如预购买产品请登陆ScienCell唯一官网(www.sciencellonline.com)寻找正规代理商,以确保您的实验顺利进行。 星形胶质细胞转染试剂盒 货号0943 产品介绍 The delivery of foreign DNA into eukaryotic cells is one of the most common molecular biology techniques to study biological mechanisms. However, unlike transformed cell lines, the efficient transfection of primary cells can be a problem. AstroFectagen is a cationic polymer-based transfection system specifically designed and optimized for efficient transfection of primary astrocytes. Transfection with AstroFectagen can be carried out in the presence of antibiotics and serum. Instead of normal two-day transfection, an optimized one-day transfection procedure can be performed for time-saving and highly reproducible transfection. One ml of AstroFectagen reagent is sufficient for up to 250 transfections per well in 96-well plate. Storage/Handling Upon receipt, aliquot and store AstroFectagen reagent A at -20°C, avoid repeated freezing/thawing cycles. Once thawed, store AstroFectagen reagent A at 4°C and use in a month. AstroFectagen reagent B can be kept at 4°C. Quality Control Each lot of AstroFectagen is performance tested by transfecting Human Astrocytes (HAs, Cat. No. 1800, ScienCell™) with PromegaR ρSV-bata-Galactosidase control vector. Gene expression is assayed by X-gal staining 24 hours post transfection. Typically, 20-45% transfection efficiency can be achieved (Figure 1). |