英文名称： ribo FECT ™ CP Transfection
riboFECT™ CP转染试剂是一种专门用于核酸分子的转染试剂，极具生物相容性，转染效率高，细胞毒性小，适合各种核酸分子(如siRNA、miRNA、质粒)的转染实验。该转染试剂转染时无需进行培养基更换操作，尤其适合各种高通量细胞筛选实验，方便，快速，高效。
| 产品编号 | 产品名称 | 价格 |
| C10511-05 | ribo FECT ™ CP Transfection Kit (166T) | ￥950 |
| C10511-1 | ribo FECT ™ CP Transfection Kit (333T) | ￥1780 |
产品描述
riboFECT™ CP转染试剂是一种专门用于核酸分子的转染试剂，极具生物相容性，转染效率高，细胞毒性小，适合各种核酸分子(如siRNA、miRNA、质粒)的转染实验。该转染试剂转染时无需进行培养基更换操作，尤其适合各种高通量细胞筛选实验，方便，快速，高效。
产品特点
■ 高转染效率，适用于大多数细胞的转染实验
■ 低细胞毒性，助您获得可靠的生理学数据
■ 操作简单方便，可反向转染，适合高通量筛选实验
-----高转染效率，低细胞毒性，只为更佳的基因沉默效率
转染效率对基因沉默实验的重要性是毋庸置疑的。riboFECT™ CP 转染试剂极具生物相容性，能够显著提高siRNA，miRNA的转染效率，为成功进行RNAi实验提供保证，并能够降低各种核酸分子最佳使用浓度，目前已 成功应用于各种真核细胞系的核酸转染，如HeLa，A549，293T，NIH/3T3，HepG2，HUVEC等细胞系。
图1 分别用riboFECT™ CP转染HUVEC（A）和Hela（B）细胞，采用riboMONITOR™检测转染效率
图2 用riboFECT™ CP和Reagent L分别转染p65 siRNA于HUVEC和Hela细胞，采用qPCR方法检测其基因沉默效率
结果显示riboFECT™ CP转染效果更好，且随着转染试剂用量升高，转染效果越好，基因沉默效率也进一步提高。
------操作流程简单，可反向转染，非常适合高通量筛选研究
仅需简单地将siRNA或miRNA与buffer混合后，加入riboFECT™ CP形成复合物，室温孵育0~10分钟，再加入一定体积的培养液，随后加入完全细胞培养基中即可，中途无需更换培养基。
反向转染实验中，先将siRNA或miRNA与riboFECT™ CP混合，加至培养孔以形成转染复合物，再将细胞加入至培养孔中进行反向转染，以实现细胞高通量筛选。其中，转染混合物制备后可保存较长时间，即允许提前准备和储存，非常适合自动化高通量筛选系统。
------可高效转染质粒，尤其适合质粒共转实验
仅需少量的riboFECT™ CP即可实现质粒的高效转染，低毒性，对细胞伤害小，对大多数细胞转染均无需更换培养液，尤其适合进行质粒共转实验，例如miRNA靶基因双荧光素酶检测实验。
图4 采用1µl riboFECT™ CP分别转染EGFP-C1（A）和EGFP-N1（B）于Hela细胞，24h后检测其表达效率
实用案例
| 转染试剂相关客户文章 | |||
| 编号 | 文章 | 杂志 | 转染细胞类型 |
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| MicroRNA-19b promotes macrophage cholesterol accumulation and aortic atherosclerosis by targeting ATP-binding cassette transporter A1 | Atherosclerosis | HEK293T |
| 1 | Let-7a inhibits migration, invasion and epithelial-mesenchymal transition by targeting HMGA2 in nasopharyngeal carcinoma | Journal of Translational Medicine | CNE-2、5-8F |
| 3 | *** attenuates advanced glycation endproducts-induced toxicity of Schwann cells in vitro. | Neurochem Res | SC |
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| MicroRNA-145 contributes to enhancing radiosensitivity of cervical cancer cells | FEBS letters | HeLa |
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| The microRNAs in the pathogenesis of metabolic memory | Endocrinology | HAEC |
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| The expression of vesicular glutamate transporter 3 and vesicular monoamine transporter 2 induced by brain-derived neurotrophic factor in dorsal root ganglion neurons in vitro | Brain research bulletin | DRG |
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| MicroRNA-101 protects cardiac fibroblasts from hypoxia-induced apoptosis via inhibition of the TGF-β signaling pathway | The International Journal of Biochemistry & Cell Biology | CF |
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| Diosgenin inhibits atherosclerosis via suppressing the MiR-19b-induced downregulation of ATP-binding cassette transporter A1 | Atherosclerosis | THP-1/MPM-derived foam cells |
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| Integrated analysis of differential miRNA and mRNA expression profiles in human radioresistant and radiosensitive nasopharyngeal carcinoma cells | PloS one | CNE2-IR |
| 3 | Proteomic and bioinformatic analysis of differentially expressed proteins in denervated skeletal muscle | Int J Mol Med | L6 skeletal muscle cells |
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| MiR-193a-3p promotes the multi-chemoresistance of bladder cancer by targeting the HOXC9 gene | Cancer Lett. | 5637、H-bc |
| 4 | MiR-9a-5p regulates proliferation and migration of hepatic stellate cells under pressure through inhibition of Sirt1 | World J Gastroenterol. | HSC |
| 5 | TRAF6 inhibition rescues ***-induced muscle atrophy | International Journal of Molecular Science | C2C12 |
| 6 | Sodium fluoride induces apoptosis through reactive oxygen species-mediated endoplasmic reticulum stress pathway in Sertoli cells | Chinese Academy of Sciences | Sertoli |
| 7 | Fluid Shear Stress Upregulates E-Tmod41 via miR-23b-3p and Contributes to F-Actin Cytoskeleton Remodeling during Erythropoiesis | PloS one | MEL |
| 8 | KITLG is a novel target of miR-34c that is associated with the inhibition of growth and invasion in colorectal cancer cells | J Cell Mol Med. | HCT-116 |
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| MicroRNA-21 Negatively Regulates Treg Cells Through a TGF-β1/Smad-Independent Pathway in Patients with Coronary Heart Disease | Cell Physiol Biochem | PBMC |
| 10 | miR-193a-3p regulates the multi-drug resistance of bladder cancer by targeting the LOXL4 gene and the Oxidative Stress pathway | Mol Cancer | 5637、H-bc |
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| The DNA methylation-regulated miR-193a-3p dictates the multi-chemoresistance of bladder cancer via repression of SRSF2/PLAU/HIC2 expression | Cell Death Dis | 5638、H-bc |
| 11 | Nestin overexpression promotes the embryonic development of heart and brain through the regulation of cell proliferation. | Brain Res | NSC |
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| MiRNA‐30a inhibits AECs‐II apoptosis by blocking mitochondrial fission dependent on Drp‐1 | J Cell Mol Med. | A549 |
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| Intercellular Adhesion Molecule-1 Inhibits Osteogenic Differentiation of Mesenchymal Stem Cells and Impairs Bio-Scaffold-Mediated Bone Regeneration In Vivo | TISSUE ENGINEERING: Part A | MSC |
| 9 | Characterization of Cep85–a new antagonist of Nek2A that is involved in the regulation of centrosome disjunction | J Cell Sci. | U2OS |
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| BRCC3 acts as a prognostic marker in nasopharyngeal carcinoma patients treated with radiotherapy and mediates radiation resistance in vitro | Radiation Oncology | HNE1、CNE2R |
*编号为实验图对应号码
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| 图1. Wu et al. Journal of Translational Medicine (2015) 13:105 | 图2. Yu et al. Neurochem Res (2015) 40:698–712 | 图3. Sun et al. Int J Mol Med (2014) 33: 1586-1596 | |
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| 图4. Lv et al. Cancer Letters 357 (2015) 105–113 | 图5. Qi et al. World J Gastroenterol (2015) 21(34): 9900-9915 | 图6. Sun et al.Int J Mol Sci(2014) 15:11126-11141 | |
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| 图7. Yang et al. J Environ Sci (2015) 30:81-89 | 图8. Mu et al. PLoS ONE (2015) 10(8): e0136607 | 图9. Chen et al. Journal of Cell Science (2015) 128, 3290-3303 | |
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| 图10. Deng et al. Molecular Cancer (2014) 13:234 | 图11. Liu et al. Brain Research (2015) 1-11 | ||
