促肾上腺皮质素释放激素结合蛋白(CRHBP)检测试剂盒(酶联免疫吸附试验法)
英文名称: ELISA Kit for Corticotropin Releasing Hormone Binding Protein (CRHBP)
型号:null    产品货号: CKRG3995
价格:请致电:010-57128832,18610462672
品牌: 中美合资

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Corticotropin Releasing Hormone Binding Protein (CRHBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Corticotropin Releasing Hormone Binding Protein (CRHBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Corticotropin Releasing Hormone Binding Protein (CRHBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Corticotropin Releasing Hormone Binding Protein (CRHBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.