IL3RA / CD123 ELISA kit, Human ELISA kit
英文名称: IL3RA / CD123 ELISA Pair Set, Human ELISA Pair Set
型号:null    抗体货号: SEK10518
价格:请致电:010-57128832,18610462672
品牌: 北京    产品商标: 义翘神州(sinobiologi

官方网址:http://www.sinobiological.cn/IL3RA-CD123-ELISA-Pair-Set-g-6728.html

Human IL3RA / CD123 ELISA Kit

Order or Inquire for IL3RA / CD123 ELISA Kit
High affinity Human IL3RA / CD123 ELISA Kit
Detection limit - 31.25 pg/ml
Affordable price and 30%-80% cost saving for Bulk order
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  • Materials
  • Protocol
  • Trouble Shooting
  • Image & Flash
     

Problems

Possible Sources

Solutions

No signal

Incorrect or no Detection Antibody was added

Add appropriate Detection Antibody and continue

Substrate solution was not added

Add substrate solution and continue

Incorrect storage condition

Check if the kit is stored at recommended condition and used before expiration date

     

Poor Standard Curve

Standard was incompletely reconstituted or was inappropriately stored

Aliquot reconstituted standard and store at  -70 ℃

Imprecise / inaccurate pipetting

Check / calibrate pipettes

Incubations done at inappropriate temperature, timing or agitation

Follow the general ELISA protocol

Background wells were contaminated

Avoid cross contamination by using the sealer appropriately

     

Poor detection value

The concentration of antigen in samples was too low

Enriching samples to increase the concentration of antigen

Samples were ineffective

Check if the samples are stored at cold environment. Detect samples in timely manner

     

High Background

Insufficient washes

Use multichannel pipettes without touching the reagents on the plate

Increase cycles of washes and soaking time between washes

TMB Substrate Solution was contaminated

TMB Substrate Solution should be clear and colorless prior to addition to wells

Materials were contaminated

Use clean plates, tubes and pipettes tips

     

Non-specificity

Samples were contaminated

Avoid cross contamination of samples

The concentration of samples was too high

Try higher dilution rate of samples

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Human IL3RA / CD123 ELISA Kit Related Products & Topics

Related Areas:

Cancer>>Cancer Stem Cell (CSC)>>CD123/IL3RA

Immunology>>Cytokine & Receptor>>Interleukin & Receptor>>Other>>CD123/IL3RA

Immunology>>Cluster of Differentiation>>T Cell CD Antigen>>Helper T Cells>>CD123/IL3RA

Proteins:

Molecule Species Description //For Detailed Info. and Price------CLICK! Cat No
CD123/IL3RA Human CD123/IL3RA/Fc Protein, Recombinant 10518-H03H
CD123/IL3RA Human CD123/IL3RA Protein, Recombinant 10518-H08H

Antibodies:

Molecule Application Description //For Detailed Info. and Price------CLICK! Cat No
Human
CD123/IL3RA
ELISA CD123/IL3RA Antibody, Rabbit MAb 10518-R012
Human
CD123/IL3RA
WB CD123/IL3RA Antibody, Rabbit MAb 10518-R017

Background

Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily. The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. The WSXWS motif of IL3RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box one motif of IL3RA is required for JAK interaction and / or activation. IL3RA represents a unique marker for primitive leukemic stem cells. Targeting of IL3RA may be a promising strategy for the preferential ablation of AML cells. Aberrant IL3RA expression is a good marker for monitoring of minimal residual disease. IL3RA is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. Recent studies have shown that interleukin-3 receptor alpha (CD123) is highly expressed on leukemia stem cells of patients with acute myeloid leukemia, and is correlated with tumor load and poor prognosis. CD123 was highly expressed in the bone marrow of the patients with myelodysplastic syndrome (MDS), significantly correlated with the proportion of bone marrow blasts, and thus might be the marker of MDS malignant clone. IL3RA is also a useful new marker for distinguishing B-cell disorders with circulating villous lymphocytes as its expression is characteristic of typical hairy cell leukemia (HCL) with high sensitivity and specificity.