1. Materials provided
Capture Antibody 0.35 mg/mL of mouse anti-IL3RA monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating.
Detection Antibody 0.5 mg/mL rabbit anti-IL3RA monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody diluteion buffer before use.
Standard Each vial contains 65 ng of recombinant IL3RA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -70℃ in a manual defrost freezer . A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2 ng/mL is recommended.
2. Sensitivity
The minimum detectable dose of Human IL3RA / CD123 was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
3. Principle of the product
The Human IL3RA / CD123 ELISA kit is for the quantitative determination of Human IL3RA / CD123.
This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on five 96-well plates.
The Sino Biological ELISA kit is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IL3RA / CD123 coated on a 96-well plate. Standards and samples are added to the wells, and any IL3RA / CD123 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-IL3RA / CD123 monoclonal antibody is then added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of IL3RA / CD123 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
STORAGE - Detection Antibody should be protected from prolonged exposure to light. Aliquot the reagents and store at -20℃ to -70℃ in a manual defrost freezer.
Human IL3RA / CD123 ELISA Kit Image

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Human IL3RA / CD123 ELISA Kit Flash
This flash is a vivid summary of the assay procedure.
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