AnnexinV Alexa Fluor647/PI 凋亡检测试剂盒
英文名称： AnnexinV Alexa Fluor647/PI 凋亡检测试剂盒
型号:null    产品货号： FXP023-100
价格:请致电：010-57128832,18610462672
品牌: 中国

                                                                                                  
封闭：20%小牛血清                                                                                                   
          4℃过夜；                                                                                                        
三、        ELISA方法（1:3500包被）                                                                                          
在酶标包被板中每孔加入100微升样品稀释液②；                                                         
在阴阳性对照孔中加入100微升对应阴性对照④，阳性对照⑤；                                     
在待测样品孔中每孔加入5微升待测样品，置37℃，30min甩出，洗板5次（洗液一袋用500ml去离子水稀释）；                                                                               
每孔加入酶结合物③100微升，30min甩出，洗板5次；                                                 

每孔加入底物液1滴，显色剂1滴，10min，每孔加入终止液1滴；