戊肝 狂犬 破伤风抗原
英文名称： antigen戊肝 狂犬 破伤风重组抗原
型号:null    抗体货号： BCW101****
价格:请致电：010-57128832,18610462672
品牌: 中国郑州    产品商标: biocell antibody

 

破伤风抗原                               
（Lockjaw-Ag）                                                  
一、        技术参数：（0.1微克可检测1人份）                                                                          
含量：3.5mg/ml                                                                                                         
纯度：≥95%                                                                                                             
存放液：PH7.4   PBS—40%甘油                                                                                   
二、        建议使用方法：（1:3000—1:4000稀释使用）                                                               
包被：PH9.6 0.05M碳酸盐缓冲液                                                                               
          4℃过夜；                                                                                                        
封闭：20%小牛血清                                                                                                   
          4℃过夜；                                                                                                        
三、        ELISA方法（1:3500包被）                                                                                          
在酶标包被板中每孔加入100微升样品稀释液②；                                                         
在阴阳性对照孔中加入100微升对应阴性对照④，阳性对照⑤；                                     
在待测样品孔中每孔加入5微升待测样品，置37℃，30min甩出，洗板5次（洗液一袋用500ml去离子水稀释）；                                                                               
每孔加入酶结合物③100微升，30min甩出，洗板5次；                                                 

每孔加入底物液1滴，显色剂1滴，10min，每孔加入终止液1滴；