性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
• 存储溶液Preservative: None
  Constituents: Whole serum
• 纯度Whole antiserum
• 克隆多克隆
• 同种型IgG
• 研究领域
  • Neuroscience
   
  • Cell Type Marker
   
  • Neuron marker
   
  • Soma marker

  • Tags & Cell Markers
   
  • Cell Type Markers
   
  • Neuroscience Markers
   
  • Neuronal

  • Epigenetics and Nuclear Signaling
   
  • Transcription
   
  • Domain Families
  • Forkhead Box
   
  • Other FOXes

  • Epigenetics and Nuclear Signaling
   
  • Transcription
   
  • Transcription Factors

应用

靶标

• 功能RNA-binding protein that regulates alternative splicing events.
• 序列相似性Contains 1 RRM (RNA recognition motif) domain.
• 细胞定位Nucleus. Cytoplasm.
• Information by UniProt
• 数据库链接
  • Entrez Gene: 146713 Human
  • Entrez Gene: 52897 Mouse
  • Entrez Gene: 287847 Rat
  • SwissProt: A6NFN3 Human
  • SwissProt: Q8BIF2 Mouse
  • Unigene: 135229 Human
  • Unigene: 341103 Mouse
  • Unigene: 143966 Rat
• 别名
  • FLJ56884 antibody
  • FLJ58356 antibody
  • Fox-1 homolog C antibody

  see all

Anti-NeuN antibody - Neuronal Marker 图像

• 

  Immunohistochemistry (Frozen sections) - Anti-NeuN antibody (ab104225)This image is courtesy of an anonymous Abreview

  ab104225 staining NeuN in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with Triton-X and blocked with 5% serum for 1 hour at 26°C. Samples were incubated with primary antibody (1/500 in blocking solution) for 12 hours at 4°C. An Alexa Fluor® 568-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody (ab104225)Carl Hobbs, King`s College London, United Kingdom

  IHC-P image of FOX3/NeuN staining using rat DRG using ab104225 (1:500). The sections were subjected to heat mediated antigen retrieval using citric acid pH 6. The sections were blocked using 1% BSA for 10 mins at 21°C. ab104225 was incubated for 2 hours at 21°C. The secondary antibody used was Goat polyclonal to Rabbit IgG conjugated to biotin (1:200).

  See Abreview

• 

  Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-NeuN antibody - Neuronal Marker (ab104225)

  ab104225 at 1/1000 dilution, staining FOX3 (red) in rat brain cortex and counterstained for DNA in blue.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody (ab104225)Carl Hobbs, King`s College London, United Kingdom

  IHC-P image of FOX3/NeuN staining using mouse DRG using ab104225 (1:500). The sections were subjected to heat mediated antigen retrieval using citric acid pH 6. The sections were blocked using 1% BSA for 10 mins at 21°C. ab104225 was incubated for 2 hours at 21°C. The secondary antibody used was Goat polyclonal to Rabbit IgG conjugated to biotin (1:200).

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody (ab104225)

  ab104225 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab104225 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.