- 经测试应用WB, IHC-FrFl, IHC-Fr, IHC-P, ICC/IF, IHC-FoFrmore details
- 种属反应性
与反应: Mouse, Rat, Human 预��可用于: Chimpanzee, Rhesus monkey, Orangutan  - 免疫原
Recombinant fragment corresponding to Human NeuN aa 1-100 (N terminal).
Sequence: MAQPYPPAQYPPPPQNGIPAEYAPPPPHPTQDYSGQTPVPTEHGMTLYTP AQTHPEQPGSEASTQPIAGTQTVPQTDEAAQTDSQPLHPSDPTEKQQPKR - 阳性对照
- Rat brain cortex tissue This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: SKNSH
性能 - 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
- 存储溶液Preservative: None
Constituents: Whole serum - 纯度Whole antiserum
- 克隆多克隆
- 同种型IgG
- 研究领域
应用Our Abpromise guarantee covers the use of ab104225 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. | 应用 | Abreviews | 说明 | | WB | | 1/5000 - 1/10000. Predicted molecular weight: 38 kDa. | | IHC-FrFl | | Use at an assay dependent concentration. | | IHC-Fr | | Use at an assay dependent concentration. | | IHC-P | | 1/500 - 1/1000. | | ICC/IF | | 1/500 - 1/1000. | | IHC-FoFr | | Use at an assay dependent concentration. | 靶标 - 功能RNA-binding protein that regulates alternative splicing events.
- 序列相似性Contains 1 RRM (RNA recognition motif) domain.
- 细胞定位Nucleus. Cytoplasm.
- Information by UniProt
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数据库链接 -
别名 - FLJ56884 antibody
- FLJ58356 antibody
- Fox-1 homolog C antibody
see all Anti-NeuN antibody - Neuronal Marker 图像 -
 Immunohistochemistry (Frozen sections) - Anti-NeuN antibody (ab104225)This image is courtesy of an anonymous Abreview ab104225 staining NeuN in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with Triton-X and blocked with 5% serum for 1 hour at 26°C. Samples were incubated with primary antibody (1/500 in blocking solution) for 12 hours at 4°C. An Alexa Fluor® 568-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. See Abreview -
 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody (ab104225)Carl Hobbs, King`s College London, United Kingdom IHC-P image of FOX3/NeuN staining using rat DRG using ab104225 (1:500). The sections were subjected to heat mediated antigen retrieval using citric acid pH 6. The sections were blocked using 1% BSA for 10 mins at 21°C. ab104225 was incubated for 2 hours at 21°C. The secondary antibody used was Goat polyclonal to Rabbit IgG conjugated to biotin (1:200). See Abreview -
 Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-NeuN antibody - Neuronal Marker (ab104225) ab104225 at 1/1000 dilution, staining FOX3 (red) in rat brain cortex and counterstained for DNA in blue. -
 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody (ab104225)Carl Hobbs, King`s College London, United Kingdom IHC-P image of FOX3/NeuN staining using mouse DRG using ab104225 (1:500). The sections were subjected to heat mediated antigen retrieval using citric acid pH 6. The sections were blocked using 1% BSA for 10 mins at 21°C. ab104225 was incubated for 2 hours at 21°C. The secondary antibody used was Goat polyclonal to Rabbit IgG conjugated to biotin (1:200). See Abreview -
 Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody (ab104225) ab104225 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab104225 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. |
