• 经测试应用IHC-FoFr, Flow Cyt, IHC-Fr, IHC-P, WB, ICC/IFmore details
• 种属反应性
  与反应: Mouse, Rat, Sheep, Goat, Cat, Dog, Human, Zebrafish, Marmoset (common)
• 免疫原

  Synthetic peptide within Human NeuN aa 1-100 (Cysteine residue). The exact sequence is proprietary.
  Database link: A6NFN3

  Run BLAST with Run BLAST with 
• 阳性对照
  • This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Mouse Cerebellum; Rat cerebellum; Human Fetal brain as well as the following whole cell lysates: 293T, HeLa and SH-SY5Y lysates; SH-SY-5Y cells. Flow Cyt: U-87MG cells.
• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Alternative versions available:

  Anti-NeuN antibody (Alexa Fluor^® 488) [EPR12763] - Neuronal Marker (ab190195)

  Anti-NeuN antibody (Alexa Fluor^® 647) [EPR12763] - Neuronal Marker (ab190565)

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存储溶液pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
• 浓度Batch dependent within range: 100 µl 浓度为 0.727 - 0.789 mg/ml40 µl 浓度为 0.727 mg/ml 10 µl 浓度为 0.727 mg/ml 
  

  查找您批次的浓度
• 纯度Protein A purified
• 克隆单克隆
• 克隆编号EPR12763
• 同种型IgG
• 研究领域
  • Neuroscience
   
  • Cell Type Marker
   
  • Neuron marker
   
  • Soma marker

  • Tags & Cell Markers
   
  • Cell Type Markers
   
  • Neuroscience Markers
   
  • Neuronal

  • Epigenetics and Nuclear Signaling
   
  • Transcription
   
  • Domain Families
  • Forkhead Box
   
  • Other FOXes

  • Epigenetics and Nuclear Signaling
   
  • Transcription
   
  • Transcription Factors

应用

靶标

• 功能RNA-binding protein that regulates alternative splicing events.
• 序列相似性Contains 1 RRM (RNA recognition motif) domain.
• 细胞定位Nucleus. Cytoplasm.

• Anti-NeuN antibody [EPR12763] - Neuronal Marker 图像

  • 

    Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

    ​An independent comparison of commercially available NeuN clones in IHC-Fr (acetone-fixed mouse dentate gyrus sections)

     

    Competitor A: Leading mouse monoclonal

    Competitor B: Non-Abcam rabbit monoclonal

     

     

    ab177487 produces intense, specific staining with minimal background, even at half the dilution of competing antibodies.

  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

    IHC image of NeuN (ab177487) with Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866) staining in formalin fixed paraffin embedded normal human cerebellum tissue section.

    The section was dewaxed and then pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with rabbit monoclonal antibody [EPR12763] to NeuN (ab177487, 0.1µg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. Endogenous peroxidases were quenched using 1.6% (v/v) hydrogen peroxide in TBS containing 0.025% (v/v) Triton X-100 for 30 minutes at room temperature, with agitation. The secondary antibody, Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866, 1.0µg/ml) was then applied for 1 hour at room temperature in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA before being developed for 10 minutes at room temperature using Steady DAB/Plus (ab103723). The section was then counterstained with hematoxylin and mounted with DPX.

    The negative control (secondary antibody only, no primary) inset shows no staining, demonstrating secondary antibody specificity.

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN [EPR12763] antibody - Neuronal Marker (ab177487)Image courtesy of Carl Hobbs (Kings College London, United Kingdom)

    IHC-P image of NeuN (green) and GFAP (red) double staining on mouse cerebellum sections using ab177487 (1/5000) and ab4674 (1/1500) respectively. The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were then incubated with Rabbit Monoclonal to NeuN (ab177487) diluted at 1/5000 and Chicken Polyclonal to GFAP (ab4674) diluted at 1/1500. The primary antibody was detected using ab150097 Goat anti-rabbit IgG conjugated to Alexa Fluor^® 488 (1/500) andab150176 Goat anti-chicken IgY conjugated to Alexa Fluor^® 594 (1/500)

  • 

    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)Image courtesy Carl Hobbs (Kings College London, United KIngdom)

    IHC-FoFr staining of NeuN staining on mouse pons sections using ab177487 (1/6000). The mouse was perfusion fixed using formaldehyde and 20µm sections were permeabilized with 0.5% tween. Blocking was performed using 1% BSA. ab177487 was diluted 1/6000 and incubated with the sections for 16 hours at 21°C. secondary antibody used was goat polyclonal to rabbit IgG conjugated to Alexa Fluor^® 594 (1/1000).

    See Abreview

  • 

    Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)Image courtesy Ryan Macdonald (Cambridge University, United Kingdom)

    IHC-Fr staining of NeuN on zebrafish brain at 4dpf sections using ab177487 (1/100). The sections were fixed in paraformaldehyde and permeabilized using triton X. Antigen retrieval uisng sodium citrate was used. The sections were blocked using 5% BSA for 1 hour at 23°C. ab177487 was diluted 1/100 and incubated for 16 hours at 4°C. The secondary antibody used was anti rabbit IgG conjugated to Alexa Fluor^® 488 (1/1000). Dapi used as counterstain.

    See Abreview

  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

    ​An independent comparison of commercially available NeuN clones in IHC-P

    
    Competitor A: Leading mouse monoclonal

    Competitor B: Non-Abcam rabbit monoclonal

     

    Sodium citrate was used for antigen retrieval in all 3 samples.

     

    ab177487 produces specific staining, equivalent to the leading mouse monoclonal at half the dilution. The non-Abcam mouse monoclonal was less specific as it stained Purkinje cells, which do not express NeuN.

  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling NeuN with unpurified ab177487 at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling NeuN with purified ab177487 at 1/3000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN [EPR12763] antibody - Neuronal Marker (ab177487)Image courtesy of Carl Hobbs (King'c College London, United Kingdom)

    IHC-P image of FOX3/NeuN staining on dog cerebellum sections using ab177487 (1/500). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10mins at 21°C. ab177487 was diluted 1/500 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to Biotin (1/250).

  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN [EPR12763] antibody - Neuronal Marker (ab177487)Image courtesy of Carl Hobbs (King'c College London, United Kingdom)

    IHC-P image of FOX3/NeuN staining on rat brain (SVZ) sections using ab177487 (1/2000). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10mins at 21°C. ab177487 was diluted 1/2000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to Biotin (1/250).

  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN [EPR12763] antibody - Neuronal Marker (ab177487)Image courtesy of Carl Hobbs (King'c College London, United Kingdom)

    IHC-P image of FOX3/NeuN staining on mouse brain (frontal cortex) sections using ab177487 (1/800). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10mins at 21°C. ab177487 was diluted 1/800 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to Biotin (1/250).

  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN [EPR12763] antibody - Neuronal Marker (ab177487)Image courtesy of Carl Hobbs (King'c College London, United Kingdom)

    IHC-P image of FOX3/NeuN staining on zebrafish spinal cord sections using ab177487 (1/500). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10mins at 21°C. ab177487 was diluted 1/500 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to Biotin (1/250).

  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN [EPR12763] antibody - Neuronal Marker (ab177487)Image courtesy of Carl Hobbs (King'c College London, United Kingdom)

    IHC-P image of FOX3/NeuN staining on marmoset cerebellum sections using ab177487 (1/2000). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10mins at 21°C. ab177487 was diluted 1/2000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to Biotin (1/250).

  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN [EPR12763] antibody - Neuronal Marker (ab177487)Image courtesy of Carl Hobbs (King'c College London, United Kingdom)

    IHC-P image of FOX3/NeuN staining on goat cerebellum sections using ab177487 (1/500). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10mins at 21°C. ab177487 was diluted 1/500 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to Biotin (1/250).

  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN [EPR12763] antibody - Neuronal Marker (ab177487)Image courtesy of Carl Hobbs (King'c College London, United Kingdom)

    IHC-P image of FOX3/NeuN staining on cat cerebellum sections using ab177487 (1/1000). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10mins at 21°C. ab177487 was diluted 1/1000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to Biotin (1/250).

  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN [EPR12763] antibody - Neuronal Marker (ab177487)Image courtesy of Carl Hobbs (King'c College London, United Kingdom)

    IHC-P image of FOX3/NeuN staining on sheep brain (Frontal cortex) sections using ab177487 (1/1000). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10mins at 21°C. ab177487 was diluted 1/1000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to Biotin (1/250).

  • 

    Immunohistochemistry (Frozen sections) - Anti-NeuN [EPR12763] antibody (ab177487)This image is courtesy of an Abreview submitted by Jianning Lu

    ab177487 staining NeuN in mouse free floating 50 micron lumbar spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with Triton X-100 and blocked with 10% serum for 2 hours at 25°C. Samples were incubated with primary antibody (1/500 in PBS + Triton) for 16 hours at 4°C. An Alexa Fluor^® 594-conjugated donkey anti-rabbit IgG polyclonal (1/700) was used as the secondary antibody.

    See Abreview

  • 

    Immunohistochemistry (Frozen sections) - Anti-NeuN [EPR12763] antibody - Neuronal Marker (ab177487)This image is courtesy of an Abreview submitted by Eva Borger

    ab177487 staining NeuN in mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with Triton X-100 + 0.4% horse seurm for 30 minutes at 20°C. Samples were incubated with primary antibody (1/500 in blocking solution) for 16 hours at 4°C. An Alexa Fluor^®594-conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • 

    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)This image is courtesy of an anonymous Abreview

    ab177487 staining NeuN in Mouse embryonic day 15 brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.5% Triton X-100 in PBS, blocked with 10% serum for 1 hour at 25°C and antigen retrieval was by heat mediation in citrate buffer, pH 6. The sample was incubated with primary antibody (1/500 in PBS + 1% serum + 0.1% Triton X-100) for 16 hours at 25°C. An Alexa Fluor^®594-conjugated Donkey anti-rabbit IgG (H+L) polyclonal (1/700) was used as the secondary antibody.

    See Abreview

  • 

    Western blot - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    All lanes : Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487) at 1/1500 dilution (unpurified)
    
    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Rat brain tissue lysate
    
    Lysates/proteins at 20 µg per lane.
    
    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
    
    Predicted band size : 34 kDa
    Observed band size : 46-48 kDa (why is the actual band size different from the predicted?)
    

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • 

    Western blot - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    All lanes : Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487) at 1/10000 dilution (purified)
    
    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Rat brain tissue lysate
    
    Lysates/proteins at 20 µg per lane.
    
    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
    
    Predicted band size : 34 kDa
    Observed band size : 46-48 kDa (why is the actual band size different from the predicted?)
    

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • 

    Western blot - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    All lanes : Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487) at 1/1000 dilution (unpurified)
    
    Lane 1 : Fetal brain lysate
    Lane 2 : 293T lysate
    Lane 3 : HeLa lysate
    Lane 4 : SH-SY5Y lysate
    
    Lysates/proteins at 10 µg per lane.
    
    Secondary
    HRP labeled goat anti-rabbit at 1/2000 dilution
    
    Predicted band size : 34 kDa
    
  • 

    Western blot - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    All lanes : Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487) at 1 µg/ml (unpurified)
    
    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Cerebellum Mouse Tissue Lysate
    Lane 3 : Cerebellum Rat Tissue Lysate
    
    Lysates/proteins at 20 µg per lane.
    
    Secondary
    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution
    
    Predicted band size : 34 kDa
    Observed band size : 48 + 50 kDa (why is the actual band size different from the predicted?)
    

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab177487 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  • 

    Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

    Immunocytochemsitry/Immunofluorescence analysis of SH-SY5Y cells labelling NeuN (green) with unpurified ab177487 at 1/80. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

  • 

    Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

    Immunocytochemsitry/Immunofluorescence analysis of SH-SY5Y cells labelling NeuN (green) with purified ab177487 at 1/300. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

  • 

    Flow Cytometry - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

    Overlay histogram showing U-87MG cells stained with ab177487 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab177487, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150081) at 1/2000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730, 1μg/1x10⁶ cells used under the same conditions. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.