Akt is a serine, threonine protein kinase critical in cellular metabolism, glucose uptake, protein synthesis, cell proliferation, growth, apoptosis, survival, angiogenesis, migration and invasion. It acts downstream of the phosphatidylinositol 3 kinase (PI3K) and it mediates the effects of several growth factors such as platelet-derived growth factor, epidermal growth factor and insulin growth factor. It is activated by phosphorylation on Ser-473, Thr-308 and Tyr-474 and when active it phosphorylates transcription factors (FOXO1), kinases (GSK-3, Raf-1, ASK, Chk1) and other signaling proteins (Bad, MDM2). There are three Akt isoforms (Akt1, Akt2 and Akt3) which share 80% sequence identity also known as PKBa, PKBß and PKB?. Akt has been shown to have a role in metabolism, apoptosis and proliferation and therefore it has been proposed to be the candidate “Warburg Kinase”.

• 检测方法IR
• 测试
  1 x 96 well plate
• 样品类型
  Adherent cells, Suspension cells
• 检测类型Cell-based (qualitative)
• 实验步骤Multiple steps standard assay
• 种属反应性
  与反应: Mouse, Human

• 组件	1 x 96 tests
  1000X IRDye® Labeled Secondary Antibody Cocktail	1 x 24µl
  100X Mouse Anti Akt-1 Primary Antibody	1 x 120µl
  100X Rabbit Anti-Akt1 pS473 Primary Antibody	1 x 120µl
  100X Triton X-100	1 x 1.25ml
  10X Blocking Buffer	1 x 15ml
  10X Phosphate Buffered Saline	1 x 100ml
  1X Janus Green Stain	1 x 11ml
  400X Tween-20	1 x 4ml