• 偶联物HRP
• 经测试应用WBmore details
• 种属反应性
  与反应: Human
• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human GAPDH aa 250-350.
  Database link: P04406

• 阳性对照
  • WB: 293T, HeLa, HepG2, HUVEC, MCF7 or SH SY5Y cell lysates.
• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Alternative versions available:

  Anti-GAPDH antibody [EPR6256] (ab128915)

  Anti-GAPDH antibody (Biotin) [EPR6256] (ab195904)

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
• 存储溶液pH: 7.4
  Preservative: 0.1% Proclin
  Constituents: PBS, 30% Glycerol, 1% BSA
• 浓度100 µl 浓度为 0.5 mg/ml 
  
• 纯度Affinity Purified
• 克隆单克隆
• 克隆编号EPR6256
• 同种型IgG
• 研究领域
  • Neuroscience
   
  • Neurology process
   
  • Neurodegenerative disease
  • Huntington's disease

  • Neuroscience
   
  • Neurology process
   
  • Neurodegenerative disease
  • Alzheimer's disease
   
  • Other

  • Isotype/Loading Controls
   
  • Loading Controls
   
  • GAPDH

  • Signal Transduction
   
  • Metabolism
   
  • Energy Metabolism

  • Cancer
   
  • Cancer Metabolism
   
  • Metabolic signaling pathway
  • Metabolism of carbohydrates

  • Metabolism
   
  • Pathways and Processes
   
  • Metabolic signaling pathways
  • Carbohydrate metabolism

  • Metabolism
   
  • Pathways and Processes
   
  • Metabolic signaling pathways
  • Energy transfer pathways
   
  • Energy Metabolism

  • Metabolism
   
  • Types of disease
   
  • Neurodegenerative disease

应用

靶标

• 功能Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
• 通路Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
• 序列相似性Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
• 翻译后修饰S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
  ISGylated.
• 细胞定位Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.

• Anti-GAPDH antibody [EPR6256] - Loading Control (HRP) 图像

  • 

    Western blot - Anti-GAPDH [EPR6256] antibody - Loading Control (HRP) (ab185059)
    All lanes : Anti-GAPDH antibody [EPR6256] - Loading Control (HRP) (ab185059) at 1/5000 dilution
    
    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    
    Lysates/proteins at 10 µg per lane.
    
    developed using the ECL technique
    
    Performed under reducing conditions.
    
    Predicted band size : 36 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    
    
    Exposure time : 2 seconds

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab185059 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406