• 偶联物Alexa Fluor® 488. Ex: 495nm, Em: 519nm
• 经测试应用Flow Cyt, ICC/IFmore details
• 种属反应性
  与反应: Human
  预测可用于: Mouse, Rat
• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Tyrosine Hydroxylase.

• 阳性对照
  • ICC/IF: SKNSH cells. Flow Cyt: SH-SY5Y cells.
• 常规说明

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  The Alexa Fluor^® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor^® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor^® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor^® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

  Alternative versions available:

  Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)

  Anti-Tyrosine Hydroxylase antibody (HRP) [EP1533Y] (ab193083)

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• 浓度100 µl 浓度为 0.5 mg/ml 
  
• 纯度Affinity Purified
• 克隆单克隆
• 克隆编号EP1533Y
• 同种型IgG
• 研究领域
  • Neuroscience
   
  • Neurotransmitter
   
  • Biogenic Amines
   
  • Dopamine

  • Neuroscience
   
  • Neurotransmitter
   
  • Biogenic Amines
   
  • Nor***

  • Neuroscience
   
  • Cell Type Marker
   
  • Neuron marker
   
  • Soma marker

  • Cancer
   
  • Cancer Metabolism
   
  • Metabolic signaling pathway
  • Hormone biosynthesis

  • Cancer
   
  • Cancer Metabolism
   
  • Response to hypoxia

  • Metabolism
   
  • Pathways and Processes
   
  • Metabolism processes
   
  • Hypoxia

  • Metabolism
   
  • Pathways and Processes
   
  • Endocrine metabolism
  • Hormone biosynthesis

应用

靶标

• 功能Plays an important role in the physiology of adrenergic neurons.
• 组织特异性Mainly expressed in the brain and adrenal glands.
• 通路Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
• 疾病相关Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
  Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
• 序列相似性Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

• Anti-Tyrosine Hydroxylase antibody [EP1533Y] (Alexa Fluor® 488) 图像

  • 

    Flow Cytometry - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (Alexa Fluor® 488) (ab192463)

    Overlay histogram showing SH-SY5Y cells stained with ab192463 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab192463, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. 
    
    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. 
    
    This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • 

    Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (Alexa Fluor® 488) (ab192463)

    ab192463 staining Tyrosine Hydroxylase in SKNSH cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192463 at a working dilution of 1 in 50 (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor^® 594, shown in red) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).