• 经测试应用WB, IHC-Pmore details
• 种属反应性
  与反应: Mouse, Rat, Human
  预测可用于: Sheep, Chicken, Cow
• 免疫原

  Recombinant full length protein corresponding to Human 14-3-3 epsilon aa 2-254. (With tag; BC000179).
  Sequence:

  DDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKN VIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDIL DVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVA YKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDD AIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVE DENQ

  
  Database link: P62258

   

  Run BLAST with Run BLAST with 
• 阳性对照
  • Mouse brain tissue lysate; HT29, 293T and HeLa cell lysates; Human colon cancer tissue.

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存储溶液pH: 7.30
  Preservative: 0.05% Sodium azide
  Constituents: 49% PBS, 50% Glycerol
  
  Note: PBS without Mg²⁺ and Ca²⁺.
• 浓度100 µl 浓度为 0.8 mg/ml 
  
• 纯度Immunogen affinity purified
• 克隆多克隆
• 同种型IgG
• 研究领域
  • Neuroscience
   
  • Neurology process
   
  • Neural Signal Transduction

  • Signal Transduction
   
  • Adapters
   
  • Cytoplasmic

  • Neuroscience
   
  • Cell Type Marker
   
  • Neuron marker
   
  • Synapse marker

  • Stem Cells
   
  • Signaling Pathways
   
  • Wnt
   
  • 14-3-3

应用

靶标

• 功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
• 序列相似性Belongs to the 14-3-3 family.
• 细胞定位Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
• Information by UniProt
• 数据库链接
  • Entrez Gene: 417554 Chicken
  • Entrez Gene: 282125 Cow
  • Entrez Gene: 7531 Human
  • Entrez Gene: 22627 Mouse
  • Entrez Gene: 29753 Rat
  • Entrez Gene: 443300 Sheep
  • Omim: 605066 Human
  • SwissProt: Q5ZMT0 Chicken

  see all
• 别名
  • 14 3 3 E antibody
  • 14 3 3 epsilon antibody
  • 14 3 3E antibody

  • 14-3-3 protein epsilon antibody
  • 14-3-3E antibody
  • 1433E_HUMAN antibody
  • Epididymis luminal protein 2 antibody
  • FLJ45465 antibody
  • FLJ53559 antibody
  • HEL2 antibody
  • KCIP 1 antibody
  • KCIP1 antibody
  • MDCR antibody
  • MDS antibody
  • Mitochondrial import stimulation factor L subunit antibody
  • Protein kinase C inhibitor protein1 antibody
  • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon antibody
  • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon polypeptide antibody
  • Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide antibody
  • YWHAE antibody

  hide

Anti-14-3-3 epsilon antibody 图像

• 

  Immunohistochemistry (Paraffin-embedded sections) - Anti-14-3-3 epsilon antibody (ab182656)

  Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue lableing 14-3-3 epsilon with ab182656 at 1/10 dilution (left panel). Right panel is treated with the immunogen.

• 

  Western blot - Anti-14-3-3 epsilon antibody (ab182656)
  All lanes : Anti-14-3-3 epsilon antibody (ab182656) at 1/250 dilution
  
  Lane 1 : HT29 cell lysate
  Lane 2 : Mouse brain tissue lysate
  Lane 3 : 293T cell lysate
  Lane 4 : HeLa cell lysate
  
  Lysates/proteins at 30 µg per lane.
  
  Secondary
  Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution
  developed using the ECL technique
  
  Predicted band size : 29 kDa
  
  
  Exposure time : 1 second

  10% SDS PAGE