| Catalog Number: 21271 | | Gene Symbol: B-RAF, B-RAF1, RAFB1 | | Description: Anti-B-Raf Rabbit Polyclonal Antibody | | Background: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the B-RAF gene cause diseases. Inherited mutations in B-RAF cause cardiofaciocutaneous syndrome. Acquired mutations in B-RAF have been found in cancers. | | Immunogen: Purified B-Raf protein, human origin. | | Applications: ELISA, WB, IHC | Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:200 | | Concentration: 0.1mg/ml | | Host: Rabbit | | Format: Liquid | | Clonality: Polyclonal | | Isotype: | | Purity: Purified from serum | | Storage buffer: | | Preservative: no | | Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | | Species Reactivity: Recognizes B-Raf of vertebrates. | | Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles | | | | Immunohistochemistry: |  | Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with anti- B-Raf polyclonal antibody (Cat. #21271). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody | | | Publications: | 1. Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
Mod Pathol. 2013 Mar;26(3):414-20. | 2. Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733 | 3. Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
human pathology. February 2015Volume 46, Issue 2, Pages 169–175 | 4. Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570 | 5. BRAF V600E Mutation-Specific Antibody: A Review
Seminars in Diagnostic Pathology | | | |