美国产品商标: neweastbioBrand,null 抗体货号： 26129Item#, Anti-B-Raf Mouse Monoclonal Antibody<br>英文名称： Anti-B-Raf Mouse Monoclonal Antibody

B-Raf [26129]
[100 µL]

Catalog Number: 26129

Gene Symbol: B-Raf

Description: Anti-B-Raf Mouse Monoclonal Antibody

Background: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.

Immunogen: A synthetic peptide from the internal region of human B-Raf.

Applications: ELISA, WB, IHC, IP

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-B-Raf antibody recognizes B-Raf of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Western blot:

Western blot analysis of recombinant B-Raf protein. Purified his-tagged B-Raf protein (Cat. #10161) was blotted with anti-B-Raf monoclonal antibody (Cat. #26129).

Publications:

1. Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
Mod Pathol. 2013 Mar;26(3):414-20.

2. Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733

3. Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
human pathology. February 2015Volume 46, Issue 2, Pages 169–175

4. Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570

5. BRAF V600E Mutation-Specific Antibody: A Review
Seminars in Diagnostic Pathology