Anti-IgA1 (subclass specific), HRP conjugate
英文名称: Anti-IgA1 (subclass specific), HRP conjugate
型号:null    抗体货号: 21-5099
价格:请致电:010-57128832,18610462672
品牌: 美国/中国    产品商标: american research pr

上海信裕生物科技有限公司是一家集研发、生产、销售、服务于一体的国际领先生物高新技术企业,专营基因、蛋白、抗体、Elisa试剂盒、细胞生物学,分子生物学等高质量生物产品。抗体纯化/标记/免疫检测:免疫分析检测是一项应用广泛的技术,利用抗原和抗体的特异性反应进行检测的一种手段,广泛用于医学病理学、免疫组织化学、分子生物学、生物制药等领域的分析研究与技术测定。制备高特异性、高效价的抗体是实验免疫学技术的基础,抗体质量的高低将直接影响试验的成败。抗体纯化能降低非特异性本底,抗体标记能通过标记物的增强放大效应提高检测灵敏度,采用ELISA、WB、IP、IHC等不同免疫分析技术检测各种样品中特定的目的蛋白。 

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Catalog Number:  26184
Gene Symbol:  BRAF, BRAF1, RAFB1
Description:   Anti-B-Raf (V600D) Mouse Monoclonal Antibody
Background:   B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.
Immunogen:   A synthetic peptide from the internal region of B-Raf (V600D), human origin.
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   
ELISA 1:1000-1:5000 
WB 1:500-1:2000 
IHC 1:100-1:200
Concentration:    0.5 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes B-Raf V600D, but not wild-type B-Raf proteins from vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant B-raf proteins. Purified His-tagged B-Raf (V600D) protein (amino acids 513-693, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-B-Raf (V600D) monoclonal antibody (Cat. # 26184).
Immunofluorescence:   
Immunofluorescence of cells expressing B-Raf proteins with anti-B-Raf (V600D) antibody. HEK293T cells were transfected with pCDNA3-GFP-B-Raf (WT) plasmid (left column) or pCDNA3-GFP-B-Raf (V600D) plasmid (right column), then fixed and stained with anti-B-Raf (V600D) monoclonal antibody (Cat. #26184).
Publications:
1.  Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
    Mod Pathol. 2013 Mar;26(3):414-20.
2.  Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
    Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733
3.  Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
    human pathology. February 2015Volume 46, Issue 2, Pages 169–175
4.  Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
    human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570
5.  BRAF V600E Mutation-Specific Antibody: A Review
    Seminars in Diagnostic Pathology