| Catalog Number: 26182 | | Gene Symbol: BRAF, BRAF1, RAFB1 | | Description: Anti-B-Raf (V600R) Mouse Monoclonal Antibody | | Background: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers. | | Immunogen: A synthetic peptide from the internal region of B-Raf (V600R), human origin. | | Applications: ELISA, WB, IHC | Recommended Dilutions:
ELISA 1:1000-1:5000
WB 1:500-1:1500
IHC 1:100-1:200 | | Concentration: 0.9 mg/ml | | Host: mouse | | Format: Liquid | | Clonality: Monoclonal | | Isotype: Unknown | | Purity: Purified from ascites | | Storage buffer: | | Preservative: no | | Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | | Species Reactivity: Recognizes B-Raf V600R, but not wild-type B-Raf proteins. | | Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles | | | | Western blot: |  | Western blot analysis of recombinant B-raf proteins. Purified His-tagged B-Raf (V600R) protein ( lane 2) and corresponding wild type protein (lane 1) were blotted with anti-B-Raf (V600R) monoclonal antibody (Cat. # 26182). | Publications: 1. Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
Mod Pathol. 2013 Mar;26(3):414-20. 2. Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733 3. Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
human pathology. February 2015Volume 46, Issue 2, Pages 169–175 4. Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570 5. BRAF V600E Mutation-Specific Antibody: A Review
Seminars in Diagnostic Pathology |