Anti-Rab5B Rabbit Polyclonal Antibody
英文名称： Anti-Rab5B Rabbit Polyclonal Antibody
型号:null    抗体货号： 21037
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

Rab5B [21037]
[100 µL]		  
Catalog Number:  21037
Gene Symbol:  RAB5B
Description:   Anti-Rab5B Rabbit Polyclonal Antibody
Background:   : Rab5, consists of three isoforms, Rab5A, Rab5B, and Rab5C, is a small GTPase that is localized to early endosomes. It regulates the fusion between endocytic vesicles and early endosomes, as well as the homotypic fusion between early endosomes.
Immunogen:   A synthetic peptide from Rab5B
Applications:   ELISA, WB, IHC
Recommended Dilutions:   ELISA 1:100-1:5000
WB 1:100-1:2000
IHC 1:100-1:1000
Concentration:   0.2 mg/ml
Host:   Rabbit
Format:   Liquid
Clonality:   Polyclonal
Isotype:   IgG
Purity:   Purified from serum
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:   Recognizes Erb-B2 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant Erb-B2 protein. Purified His-tagged Erb-B2 protein (Cat. #10208) was blotted with anti-Erb-B2 monoclonal antibody (Cat. #26127).
Immunohistochemistry:  
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti-Erb-B2 monoclonal antibody (Cat. #26127). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.