Anti-ERBB2 Mouse Monoclonal Antibody
英文名称： Anti-ERBB2 Mouse Monoclonal Antibody
型号:null    抗体货号： 26127
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

ERBB2 [26127]
[100 µL]		  

Catalog Number:  26127
Gene Symbol:  ERBB2/HER2/Neu/c-Neu
Description:   Anti-ERBB2 Mouse Monoclonal Antibody
Background:   The HER-2/neu oncogene, a member of the epidermal growth factor receptor or erb-B gene-like family, encodes a transmembrane tyrosine kinase receptor that mediates extracellular signals activated by epidermal growth factors. Her2 abnormal has been strongly associated with many malignant tumors, especially with breast cancers. The expression level of Her2 is an important critiria in clinic evaluating of the progression of breast cancer.
Immunogen:   A synthetic peptide from the C-terminal region of ERBB2, human origin
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:500-1:2000 
IHC: 1:50-1:200
Concentration:    1mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:   Recognizes Erb-B2 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant Erb-B2 protein. Purified His-tagged Erb-B2 protein (Cat. #10208) was blotted with anti-Erb-B2 monoclonal antibody (Cat. #26127).
Immunohistochemistry:  
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti-Erb-B2 monoclonal antibody (Cat. #26127). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.