Anti-ErbB2 (V777A) mouse monoclonal Antibody
英文名称： Anti-ErbB2 (V777A) mouse monoclonal Antibody
型号:null    抗体货号： 26080
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

ERBB2 (V777A) [26080]
[100 µL]		  

Catalog Number:  26080
Gene Symbol:  ERBB2/HER2/Neu
Description:   Anti-ErbB2 (V777A) mouse monoclonal Antibody
Background:   The ErbB family is composed of four plasma membrane-bound receptor tyrosine kinases. Amplification or over-expression of the ERBB2 gene occurs in approximately 30% of breast cancers. HER2 is encoded by ERBB2, a known proto-oncogene located at the long arm of human chromosome 17(17q21-q22). HER2 proteins have been shown to form clusters in cell membranes that may play a role in tumorigenesis. Mutations of ErbB2 protein, including V777A, have been indicated in human cancers.
Immunogen:   A synthetic peptide surround the 777A codon of ErbB2 protein.
Tested Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   
ELISA: 1:500-1:5000 
WB: 1:200-1:2000 
IF: 1:100 
IHC: 1:50-1:100
Concentration:   0.2 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes V777A mutant but not wild type ErbB2 protein, of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles.
 
Immunofluorescence:  
Immunofluorescence of cells expressing ErbB2 proteins with anti-ErbB2 (V777A) antibody. HEK293T cells were transfected with pCDNA3-GFP- ErbB2 WT plasmid (left column) or pCDNA3-GFP-ErbB2 (V777A) plasmid (right column), then fixed and stained with anti-ErbB2 (V777A) monoclonal antibody (Cat. #26080).