Anti-ERBB2 (D769H) Mouse Monoclonal Antibody
英文名称：  Anti-ERBB2 (D769H) Mouse Monoclonal Antibody
型号:null    抗体货号： 26381
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

ERBB2 (D769H) [26381]
[100ul]		  

Catalog Number:  26381
Gene Symbol:  ERBB2/HER2/Neu/c-Neu
Description:   Anti-ERBB2 (D769H) Mouse Monoclonal Antibody
Background:   The HER-2/neu oncogene, a member of the epidermal growth factor receptor or erb-B gene-like family, encodes a transmembrane tyrosine kinase receptor that mediates extracellular signals activated by epidermal growth factors. Her2 abnormal has been strongly associated with many malignant tumors, especially with breast cancers. The expression level of Her2 is an important critiria in clinic evaluating of the progression of breast cancer.
Immunogen:   A synthetic peptide from the internal region of ERBB2 which includes the mutation of D769H, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:2000 
WB: 1:500-1:1000 
IF: 1:25-1:50
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes D769H mutant, but not wild-type ERBB2 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant ERBB2 (D769H) and wildtype proteins. Purified His-tagged ERBB2 (D769H) protein (lane 1) and corresponding wildtype protein (lane 2) were blotted with anti-ERBB2 (D769H) monoclonal antibody (Cat. #26381).
Immunofluorescence:  
Immunofluorescence of cells expressing ERBB2 proteins with anti-ERBB2 (D769H) antibody. HEK293T cells were transfected with pCDNA3-GFP-ERBB2 (WT) plasmid (left column) or pCDNA3-GFP-ERBB2 (D769H) plasmid (right column), then fixed and stained with anti-ERBB2 (D769H) monoclonal antibody (Cat. #26381).