Anti-ErbB2 Rabbit Polyclonal Antibody
英文名称: Anti-ErbB2 Rabbit Polyclonal Antibody
型号:null    抗体货号: 21202
价格:请致电:010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

ERBB2 [21202]
[100 µL]
 

Catalog Number:  21202
Gene Symbol:  ERBB2/HER2/Neu
Description:   Anti-ErbB2 Rabbit Polyclonal Antibody
Background:   The ErbB family is composed of four plasma membrane-bound receptor tyrosine kinases. Amplification or over-expression of the ERBB2 gene occurs in approximately 30% of breast cancers. HER2 is encoded by ERBB2, a known proto-oncogene located at the long arm of human chromosome 17(17q21-q22). HER2 proteins have been shown to form clusters in cell membranes that may play a role in tumorigenesis.
Immunogen:   A synthetic peptide from the central of ErbB2 protein.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:500-1:5000 
WB: 1:200-1:2000 
IHC: 1:20-1:200
Concentration:   0.2 mg/ml
Host:   Rabbit
Format:   Liquid
Clonality:   Polyclonal
Isotype:   
Purity:   Purified from serum
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes ErbB2 of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles.
 
Western blot:  
Western blot analysis of ErbB2 protein. Purified full length His-tagged ErbB2 protein (Cat. #10208) was blotted with anti-ErbB2 rabbit polyclonal antibody (Cat. #21202).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti-ERBB2 polyclonal antibody (Cat. #21202). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4 °C. A HRP-conjugated Goat anti-Rabbit IgG (1:50) was used as secondary antibody.