加拿大产品商标: stressmarqBrand,null 抗体货号： SPC-685DItem#, BNIP3 抗体<br>英文名称： BNIP3 Antibody

Catalog Number: 26274

Gene Symbol: C-Kit; CD117; KIT; PBT; SCFR

Description: Anti-KIT (D820E) Mouse Monoclonal Antibody

Background: Tyrosine-protein kinase Kit, also known as proto-oncogene c-Kit or CD117 or Mast/stem cell growth factor receptor (SCFR), is a receptor tyrosine kinase protein that in humans is encoded by the KIT gene. Activating mutations in this gene are associated with gastrointestinal stromal tumors, testicular seminoma, mast cell disease, melanoma, acute myeloid leukemia, while inactivating mutations are associated with the genetic defect piebaldism.

Immunogen: A synthetic peptide from the internal region of KIT which includes the mutation of D820E, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes D820E mutant, but not wild-type KIT of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Western blot:

Western blot analysis of recombinant KIT (D820E) and wild-type proteins. Purified His-tagged KIT (D820E) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with anti-KIT (D820E) monoclonal antibody (Cat. #26274).

Immunofluorescence:

Immunofluorescence of cells expressing KIT proteins with anti-KIT (D820E) antibody. HEK293T cells were transfected with pCDNA3-GFP-KIT (WT) plasmid (left column) or pCDNA3-GFP-KIT (D820E) plasmid (right column), then fixed and stained with anti- KIT (D820E) monoclonal antibody (Cat. #26274).